The goal of our project is the production of a chitosan hydrogel which uses chitin as source material. This N-acetylglucosamine oligosaccharide (chitin) can be produced in E. coli via a Chitin Synthase (CHS). The enzyme used here is CHS NodC from the bacteria Rhizobium Leguminosarum. NodC is an N-acetylglucosaminyl transferase which builds chitin tetramers and pentamers using activated N-Acetylglucosamine monomers [Samain et al., 1997]. Originally, chitin is chemically recovered from crustacean shells, which produces a lot of waste and chitin oligosaccharides of unspecified length [Kurita, 2006]. To avoid this waste, we produce chitin oligosaccharides in E. coli by insertion of the nodC gene with the BioBrick system [Knight et al., 2003]. NodC reliably produces short oligosaccharides of certain lengths which can be directly further processed in vivo.[Samain et al., 1997]
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