Team:BIT-China/Notebook
NOTEBOOK
Breakthrough
2.14-4.14: Recruiting team & Brainstorm
4.14-6.20: core part synthesis and heterologous expression
4.14-5.2 : core part-T1R2/T1R3 synthesis completely through OE-PCR
4.30-6.20 : heterologous expression of T1R2/T1R3 and characteristic
4.30-5.15：modeling
4.30-5.5：finish several sweeteners’ as well as T1R2/T1R3 PDB file construction. Different sweeteners and numbers are docking to the sweet receptor T1R2/T1R3.
5.5-5.15：MAPK pathway signal modeling
5.2-8.20: pathway optimization
5.2-7.1 : single gene knock out
7.1-8.5 : knock out gene combination
8.5-8.20 : engineered strain characterization
8.21-10.20: new pathway verification
8.21-9.13 : diction circuit establishment
9.13-9.26 : new pathway verification
9.27-10.10 : sweet meter verification
10.8-10.20 : parts standardization
For more details,please see our project.
