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InterLab Measurement Studies

iGEM is challenging the problem of different groups measuring fluorescence differently, in different units and evaluate the results non-identical. Therefore, the InterLab Study aims to develop a standardized protocol for repeatable fluorescence measurements of GFP. The study should be carried out and examined in different laboratories all over the world. Our team followed the standardized procedure guiding us through transformation, inoculation and measurement process. For more information: (LINK) Following below you can find our results.

Calibration

OD600 reference point

Several measurements of LUDOX-S40 and H²O were spectrometric analyzed below.

LUDOX-S40 H²O
Replicate 1 0,041700002 0,035300002
Replicate 2 0,042399999 0,035
Replicate 3 0,043000001 0,035700001
Replicate 4 0,043000001 0,034699999
Arithmetic Mean 0,042525001 0,035175
Corrected Absorbance 600 0,007350001
Reference OD600 0,0425
Correction Factor 5,78231249