Team:Sydney Australia/Parts

GOLD

 

GOLD

 

 

PART NAME / NO.

 

TYPE

SOURCE

KEY FEATURES

YncM-Winsulin

BBa_K2417005

COMPOSITE PART

 

Bacillus subtilis (YncM secretion tag)

 

 

·      Novel short chain insulin analogue (Winsulin) with a 12AA linker (QRGGGSGGGQRR)

·      YncM secretion tag to translocate protein into media

·      N-terminal 6x His Tag purification

·      4x GSS flexible linker

·      TEV protease cleavage site

·      Extended ribosome binding site

 

Ecotin-Proinsulin

BBa_K2417000

 

COMPOSITE PART

E. Coli (Ecotin tag)

 

·      Human proinsulin

·      N-terminal Ecotin tag for targeting proteins to the periplasm

·      N-terminal 6x His tag

·      4x GGS flexible linker

·      trypsin protease cleavage site for His-tag and C-peptide cleavage

·      Extended ribosomal binding site

 

Human (Human proinsulin)

Cytoplasmic-Proinsulin     BBa_K2417001

COMPOSITE PART

Human (Human proinsulin)

·      Human proinsulin

·      N-terminal 6x His tag

·      4x GGS flexible linker

·      Trypsin protease cleavage site for His-tag and C-peptide cleavage

·      Extended ribosomal binding site

SILVER

 

SILVER

 

 

PART NAME /  NO.

 

TYPE

SOURCE

KEY FEATURES

Cytoplasmic-Winsulin

BBa_K2417003

COMPOSITE PART

N/A – Winsulin is self designed from literature and mammalian insulins

 

 

·      Novel single chain insulin analogue (Winsulin) with a 12AA linker (QRGGGSGGGQRR)

·      N-terminal 6x His Tag

·      4x GSS flexible linker

·      TEV protease cleavage site

·      Extended ribosome binding site

 

Ecotin-Proinsulin

BBa_k2417000

COMPOSITE PART

E. Coli (Ecotin Tag)

 

·      Human proinsulin

·      N-terminal Ecotin tag for targeting proteins to the periplasm

·      N terminal  x His Tag

·      4x GSS flexible linker

·      TEV protease cleavage site

·      Extended ribosomal binding site

 

Human (Human proinsulin)

BRONZE

 

BRONZE

 

 

PART NAME / NO.

 

 

TYPE

 

SOURCE

 

KEY FEATURES

Ecotin-Winsulin

BBa_K2417002

COMPOSITE PART

 

E. Coli (Ecotin Tag)

 

·      Novel single chain insulin analogue (Winsulin) with a 12AA linker (QRGGGSGGGQRR)

·      N-terminal Ecotin tag for targeting proteins to the periplasm

·      N-terminal 6x His Tag

·      4x GSS flexible linker

·      TEV protease cleavage site

·      Extended ribosome binding site

 

N/A – Winsulin is self designed from literature and mammalian insulins