Team:Sydney Australia/Parts

GOLD

PART NAME /  NO.	TYPE	SOURCE	KEY FEATURES
YncM-Winsulin BBa_K2417005	COMPOSITE PART	Bacillus subtilis (YncM secretion tag)	Novel short chain insulin analogue (Winsulin) with a 12AA linker (QRGGGSGGGQRR) YncM secretion tag to translocate protein into media N-terminal 6x His Tag purification 4x GSS flexible linker TEV protease cleavage site Extended ribosome binding site
Ecotin-Proinsulin BBa_K2417000	COMPOSITE PART	E. Coli (Ecotin Tag)	Human proinsulin N-terminal Ecotin tag for targeting proteins to the periplasm N terminal 6x His Tag 4x GSS flexible linker TEV protease cleavage site Extended ribosomal binding site
		Human (Human proinsulin)
Cytoplasmic-Proinsulin     BBa_K2417001	COMPOSITE PART	Human (Human proinsulin)	Human proinsulin N-terminal 6x His tag 4x GGS flexible linker Trypsin protease cleavage site for His-tag and C-peptide cleavage Extended ribosomal binding site

SILVER

PART NAME /  NO.	TYPE	SOURCE	KEY FEATURES
Cytoplasmic-Winsulin BBa_K2417003	COMPOSITE PART	N/A – Winsulin is self designed from literature and mammalian insulins	Novel single chain insulin analogue (Winsulin) with a 12AA linker (QRGGGSGGGQRR) N-terminal 6x His Tag 4x GSS flexible linker TEV protease cleavage site Extended ribosome binding site
Ecotin-Proinsulin BBa_K2417000	COMPOSITE PART	E. Coli (Ecotin Tag)	Human proinsulin N-terminal Ecotin tag for targeting proteins to the periplasm N terminal 6x His Tag 4x GSS flexible linker TEV protease cleavage site Extended ribosomal binding site
		Human (Human proinsulin)

BRONZE

PART NAME / NO.	TYPE	SOURCE	KEY FEATURES
Ecotin-Winsulin BBa_K2417002	COMPOSITE PART	E. Coli (Ecotin Tag)	Novel single chain insulin analogue (Winsulin) with a 12AA linker (QRGGGSGGGQRR) N-terminal Ecotin tag for targeting proteins to the periplasm N-terminal 6x His Tag 4x GSS flexible linker TEV protease cleavage site Extended ribosome binding site
		N/A – Winsulin is self designed from literature and mammalian insulins