Team:GZHS-United/Notebook
| Date | objection | result | Analysis | Solution |
|---|---|---|---|---|
| 7-10 | 1. Extract the genomic DNA of Bt and Bs. | 1. Only the genomic DNA of Bs were obtained. | The Bt haven’t been actived. | Culture Bt in LB to active it. |
| 7-11 |
1. Extract the genomic DNA of Bt. 2. Acquire mtx gene form genomic DNA of Bs by PCR. |
1. DNA electrophoresis showed that the strip appeared at 150bp. 2. A peal DNA strip in agarose gel electrophoresis were observed. |
1. The fragment we extracted were RNA. |
1. Use a more gentle method to broke the Gram-negative bacterium cell wall. 2. Optimize the PCR condition and try to use the diluted production as template. |
| 7-12 |
1. Extract the genomic DNA of Bt. 2. Amplification of mtx1 gene. |
1. The genomic DNA of Bt were successfully obtained. 2. The mtx1 gene was successfully obtained. 
|
Boiling water-bath is a good method to broke the bacterium cell wall. | / |
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