Team:BIT-China/Notebook
NOTEBOOK
Breakthrough
2.14-4.14:Team recruitment & Brainstorm
4.14-6.20: core part synthesis and heterologous expression
4.14-5.2 : core part-T1R2/T1R3 synthesis completely through OE-PCR.
4.30-6.20 : heterologous expression of T1R2/T1R3. Click here to see more details.
4.30-5.15：modeling
4.30-5.5：finished several sweeteners’docking as well as T1R2/T1R3 PDB file construction. Different sweeteners docked to the sweet taste receptor T1R2/T1R3.Click here to see more details.
5.5-5.15：MAPK pathway signal modeling. Click here to see more details.
5.2-8.20: pathway optimization
5.2-7.1 : single gene knockout
7.1-8.5 :any two of the three genes knockout and three genes knockoutClick here to see more details.
8.5-8.20 : characterization of engineered strains
8.21-10.20:Verification of the new pathway
8.21-9.13 : establishment of the detection circuit .Click here to see more details.
9.13-9.26 : verification of the new pathway
9.27-10.10 : sweetness meter verification
10.8-10.20 : parts standardization
For more details,please see our project.
