Team:Stuttgart/Rose and Limonene Fragrance
Results
Rose and Limonene Fragrance
Rose Fragrance
The rose genes KDC-YjgB-ARO8 and ATF1 were sucessfully amplified by PCR, using plasmids pET28a-KDC-YjgB-ARO8 and pET28a-ATF1 from Guo et al. The agarosegel analysis showed expected ties in the range between 4000-5000 kDa for KDC-YjgB-ARO8 and another tie at 1600 kDa for ATF1. The arabiniose-inducable promotor pBAD (BBa_K206000 from the iGEM kit plate) could also be amplified by PCR. The purified PCR product showed a discrete tie at 130 kDa. Additionally another PCR was perfomed, to couple the pBAD promotor to the gene complex with KDC-YjgB-ARO8. The Verification by agarosegel electrophoresis showed that the coupling wasn't sucessfull.
HIER TEXT REINKOPIEREN
HIER TEXT REINKOPIEREN
HIER TEXT REINKOPIEREN
