Team:TecMonterrey GDA/Collaborations
TecMonterrey_GDA
Collaborations
CDMX team designed and developed a new method for siRNA production in BioBrick BSLA, which encodes for the reporter gene of the Blue Chromoprotein, the sense strand of the siRNA, a loop, and the antisense strand of the siRNA. As the protocol stayed E. coli HT115 was used in the presence of plasmid L440, which confers resistance to ampicillin and tetracycline. When transforming this BioBrick in pSB1C3, the bacteria is resistant to ampicilin, tetracycline, and chloramphenicol. Due to the risk of using multiresistant bacteria in research and to optimize BSLA expression, they asked Guadalajara team to determine the influence of multiple antibiotic resistance in the expression of BSLA. In order to achieve this, the received strain was cultivated in luria broth with 1) chloramphenicol, 2)chloramphenicol and ampicilin, 3) chloramphenicol and tetracycline, and 4) chloramphenicol, ampicilin, and tetracycline. A kinetic characterization of the strain was performed using nanodrop technology. Finally, the resulting biomass was then recovered and used to run an electrophoresis.
Silver Medal Criterion #2
Complete this page if you intend to compete for the silver medal criterion #2 on collaboration. Please see the 2017 Medals Page for more information.
Which other teams can we work with?
You can work with any other team in the competition, including software, hardware, high school and other tracks. You can also work with non-iGEM research groups, but they do not count towards the iGEM team collaboration silver medal criterion.
In order to meet the silver medal criteria on helping another team, you must complete this page and detail the nature of your collaboration with another iGEM team.
Here are some suggestions for projects you could work on with other teams:
- Improve the function of another team's BioBrick Part or Device
- Characterize another team's part
- Debug a construct
- Model or simulating another team's system
- Test another team's software
- Help build and test another team's hardware project
- Mentor a high-school team
