Team:ITB Indonesia/Part Collection
Parts
Parts Collection
Basic Parts
This is the parts collection designed by ITB Indonesia iGEM Team 2017.
:
| Part Name | BioBrick | Description | Part Type |
|---|---|---|---|
| PETase | BBa_K2378001 | This is optimized coding sequence of PETase enzyme for E. coli BL21. | Coding |
| IrrE Coding Sequence | BBa_K2378007 | This is improved coding sequence of IrrE from BBa_K729001 (http://parts.igem.org/Part:BBa_K729001). When transformed to E. coli, this protein protected from salt, oxidative, and thermal shock. | Coding |
| EndA Endonuclease Coding Sequence from Vibrio cholera | BBa_K2378008 | This endonuclease degrades free extracellular DNA. The gene is isolated from Vibrio cholera. | Coding |
| pSal + PETase Coding Device | BBa_K2378004 | ITB_Indonesia iGEM team 2017 designed a PETase enzyme coding device consisting of NahR-pSal regulatory part (BBa_J61051, http://parts.igem.org/Part:BBa_J61051) which is activated by salicylate inducer. In effect, PETase is only expressed when this device detect the presence of salicylate, which binds to NahR. | Composite |
| Constitutive PETase Coding Device | BBa_K2378005 | This is optimized coding sequence of PETase enzyme for E. coli BL21 with the addition of constitutive promoter BBa_J23106. | Composite |
| NhaR-PETase Constitutive Coding Device | BBa_K2378006 | This is an optimized coding sequence of PETase enzyme for E. coli BL21 with the addition of NhaR BBa_K1357002 (http://parts.igem.org/wiki/index.php?title=Part:BBa_K1357002) coding sequence. NhaR enhances the transcription of pgaABCD operon which in turn induces the production of biofilm. This part also uses constitutive promoters BBa_J23106. | Composite |
| EndA Endonuclease Coding Device from Vibrio cholera | BBa_K2378009 | This is coding device of endonuclease degrades free extracellular DNA, with constitutive promoter BBa_J61051. The gene is isolated from Vibrio cholera. | Composite |
