Testheading
OMG | WTF |
MAX MUSTERMANN align="right" |
SCHEIßE | SCHEIßE2 |
Aachen | Aalto-Helsinki | Aix-Marseille |
Bielefeld-CeBiTec | UBonn_HBRS | ColumbiaNYC |
![](https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg)
HIER DEN TEXT | ![]() |
Results and engineered constructs of artico
- By decorating the peroxisomes with the v-SNARE Snc1 we successfully secreted their entire contents
- With two different sensors we were able to efficiently measure the pH and the redox potential inside our yeast peroxisomes.
- Via fluorescence microscopy we verified that the integration of new membrane proteins into the peroxisomal membrane is possible.
- By successfully translocating the required enzymes for the metabolic pathways of Nootkatone and Violacein into the peroxisome and actually synthesizing the latter, we developed a proof of concept for our toolbox
- We successfully implemented a way of customizing the size and number of the peroxisomes into our toolbox.
- With a high throughput assay we characterized the import efficiency of different PTS2 sequences.
- To get a better understanding of possible problems and pitfalls of our metabolic engineering concepts we extensively modeled the whole nootkatone pathway and the benefits of it being translocated inside our compartment.
- For our planned optogenetic experiments we designed an affordable lightbox which can easily be assembled in a short time.
- All our excellent results can be combined into a highly variable compartment toolbox for designing artificial compartments based on the peroxisomes in S. cerevisiae with an enormous range of applications.
![](https://static.igem.org/mediawiki/2017/5/50/T--Cologne-Duesseldorf--check.jpeg)