| Reults |
Acchieved |
| We were able to design and successfully test an orthogonal peroxisomal protein import mechanism for the peroxisome in S. cerevisiae |
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| By decorating the peroxisomes with the v-SNARE Snc1 we successfully secreted their entire contents |
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| With two different sensors we were able to efficiently measure the pH and the redox potential inside our yeast peroxisomes |
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| Via fluorescence microscopy we verified that the integration of new membrane proteins into the peroxisomal membrane is possible |
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| By successfully translocating the required enzymes for the metabolic pathways of Nootkatone and Violacein into the peroxisome and actually synthesizing the latter, we developed a proof of concept for our toolbox |
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| We successfully implemented a way of customizing the size and number of the peroxisomes into our toolbox |
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| With a high throughput assay we characterized the import efficiency of different PTS2 sequences |
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| To get a better understanding of possible problems and pitfalls of our metabolic engineering concepts we extensively modeled the whole nootkatone pathway and the benefits of it being translocated inside our compartment |
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| For our planned optogenetic experiments we designed an affordable lightbox which can easily be assembled in a short time
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| All our excellent results can be combined into a highly variable compartment toolbox for designing artificial compartments based on the peroxisomes in S. cerevisiae with an enormous range of applications
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