Team:ITB Indonesia/Improve

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Improve


Improve

BBa_K2378007 is an improved coding sequence of IrrE from [1] From the alignment process, it is shown that the 'lost' protein from the stop codon is not from the peptidase domain sequence. But, from the length of the untranslated residues (~30 aa), it is hypothesized that this will affect the structural stability of the protein itself. Moreover, the second protein is not functional and does not have any motifs, hence will be possible burden for the metabolic process efficiency.

Improvement

We optimized the sequence for the expression in Escherichia coli BL21 by using synonymous mutations, then made the protein contiguous while being translated. We also removed the low complexity region.

Figure 1: Protein architecture of BBa_K729001. DUF955 is peptidase domain.
Figure 2: Protein architecture of novel BBa K2378007. DUF955 is peptidase domain.