Team:ITB Indonesia/Demonstrate

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Demonstrate

Demonstrate

Real World's Conditions, Real World's Materials

In order to demonstrate the work of our project in real world's conditions, we did not only use chemical test reagents (such as pNPB) to test our PETase activity. We instead tried to find the realest material that would be encountered by our bacteria on its applications. We ended up using plastic bottles directly as materials to be degraded.

The results of commonly found plastic bottle degradation by our bacteria are quite satisfactory. SEM analyses showed that our bacteria are able to degrade even real life plastic bottles, which are not pure PET. The effect is shown by SEM analyses

Figure 2. SEM result of plastic fragment incubated with control BL21 cells bearing no plasmid
Figure 3. SEM result of plastic fragment incubated with BL21 cells transformed with BBa_K2378005 shows rougher surface with more cracks
Figure 4. SEM result of plastic fragment incubated with BL21 cells transformed with BBa_K2378006 shows rougher surface with more cracks