Difference between revisions of "Team:KU Leuven/Experiments"
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<h1>Experiments</h1> | <h1>Experiments</h1> | ||
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| − | + | In the lab, we used different experimental procedures. There are protocols for the wet and bacterial lab, for the cell culture lab and for the electrophysiology lab. | |
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</p> | </p> | ||
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<div> | <div> | ||
| − | <h3> | + | <h3>Wet Lab and bacterial lab</h3> |
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<p style="text-align:justify"> | <p style="text-align:justify"> | ||
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<div class="header"> | <div class="header"> | ||
<div> | <div> | ||
| − | <h3> | + | <h3>Cell Culture</h3> |
| + | </div> | ||
| + | </div> | ||
| + | <div class="content" style="display: none;"> | ||
| + | <p style="text-align:justify"> | ||
| + | <b>Medium of HEK T293 cells</b> | ||
| + | <br> | ||
| + | <ul> | ||
| + | <li>DMEM (Gibco N° 41965-039) 500 ml</li> | ||
| + | <li>50 ml (10 %) FCS = (Hyclone)</li> | ||
| + | <li>10 ml Penicillin/Streptomycin (Gibco N° 15070-071) = 100-100U/ml</li> | ||
| + | <li>10 ml Glutamax (Gibco N° 35050-038) = 10 mM</li> | ||
| + | <li> 5 ml Non-Essential-Amino-Acids (Gibco N° 1140-035) = 10x</li> | ||
| + | </ul> | ||
| + | <br> | ||
| + | <br> | ||
| + | <b>Growing Conditions</b> | ||
| + | <br> | ||
| + | <ul> | ||
| + | <li>Incubate at 37°C and 10% CO2</li> | ||
| + | <li>Split cells around 70-90% confluency</li> | ||
| + | <li>Change medium twice a week</li> | ||
| + | </ul> | ||
| + | <br> | ||
| + | <br> | ||
| + | <b>Standard procedure for Trypsinization</b> | ||
| + | <br> | ||
| + | <ol type="1"> | ||
| + | <li>Wash the cells with Versene (Gibco N° 15040-033, ± 2 min)</li> | ||
| + | <li>Remove versene and add Trypsin (Gibco N° 25300-096)</li> | ||
| + | <li>Incubate at 37°C (around 3-5 min)</li> | ||
| + | <li>Stop the trypsinization by adding 5-10 times volume of medium</li> | ||
| + | <li>Count cells</li> | ||
| + | <li>Centrifuge for 5 minutes at 1200 rpm</li> | ||
| + | <li>Aspirate supernatant and resuspend the cells in medium</li> | ||
| + | <li>Plate cells for experiments or culture</li> | ||
| + | </ol> | ||
| + | |||
| + | <br> | ||
| + | <b>Coating | ||
| + | |||
| + | </p> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="experiment"> | ||
| + | <div class="header"> | ||
| + | <div> | ||
| + | <h3>Electrophysiology Lab</h3> | ||
</div> | </div> | ||
</div> | </div> | ||
Latest revision as of 13:54, 30 August 2017
Experiments
In the lab, we used different experimental procedures. There are protocols for the wet and bacterial lab, for the cell culture lab and for the electrophysiology lab.
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
