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Revision as of 14:17, 8 September 2017

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YORK
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Notebook

Home Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7 Week 8 Week 9 Week 10

Week 1 (June 2 - June 8)

  • Wet Lab Overview

    Transformation efficiency and protocols were tested - heat shock did not work well, and we came to the conclusion to stick with electroporation. The first day of Wet Lab training began on the 7th! We started working on amplification of metallothionein genes - so far nixA and GST-PMT appear to be working.

  • Dry Lab Overview

    Today was the first Dry Lab meeting for the new project! We discussed the mechanics and optimization of general filtration systems. We also started looking for the best way to make hollow fiber reactors the focal point of our filtration system.



Week 2 (June 9 - June 15)

  • Wet Lab Overview

    Obtained transformants of pA14F and pC14T heat shocks and both E/B and E/D were successfully heat shocked into DH5a. Ran into problems later in the week with heat shocked plates from overgrowth and contamination; switched to electroporating of C+F and E+B.

  • Dry Lab Overview

    We began the design process for our filtration system by choosing our ideal target: runoff streams that feed contaminated water into natural rivers. By basing our assumptions around this, we were able to sketch out some functional requirements for our system, such as minimum flow rate, power consumption, materials, etc. We divvied up the system into subparts to research and report back next week.