Difference between revisions of "Team:UChicago"
| Line 21: | Line 21: | ||
<div class="paragraph"id="p1"> | <div class="paragraph"id="p1"> | ||
| − | + | Plasmid vectors are invaluable tools for synthetic biologists and the broader scientific community. For those who work with yeast, one particularly useful vector is the centromeric plasmid: a vector that, by essentially mimicking a yeast chromosome, is able to provide both the flexibility of replicating plasmids and the stability of integrating plasmids. | |
</div> | </div> | ||
| Line 27: | Line 27: | ||
<div class="paragraph"id="p2"> | <div class="paragraph"id="p2"> | ||
| − | + | Organisms often do not retain plasmids once they have been transformed. Plasmid loss occurs when cells undergo replication. During mitosis, microtubules attach to the centromere of DNA and distribute it evenly among the daughter cells. If we were to insert a centromeric sequence into a plasmid, the microtubules would attach to this region of DNA. As a result, the plasmid would be distributed much more equally (and without loss) among the daughter cells in mitosis. | |
| − | + | ||
</div> | </div> | ||
| Line 34: | Line 34: | ||
<div class="paragraph"id="p3"> | <div class="paragraph"id="p3"> | ||
| − | + | While there are multiple centromere plasmid options for <i>Saccharomyces cerevisiae</i>, the scientific community has yet to design a yeast centromere series for the other widely-used yeast strain, <i>Pichia pastoris</i>. In response, <b>UChicago Genehackers has set out to design the first yeast centromeric plasmid for <i>Pichia pastoris</i></b>. | |
| − | + | ||
</div> | </div> | ||
| Line 41: | Line 41: | ||
<div class="paragraph"id="p3"> | <div class="paragraph"id="p3"> | ||
| − | + | We will identify the minimal, essential region of the Pichia centromere that, when incorporated into a plasmid backbone, will allow the plasmid to mimic a Pichia chromosome during replication. This region can then be incorporated into Pichia backbones to convert them into centromere plasmids, which transform with high efficiency, have low and constant copy numbers, and are stably maintained without integration. Ultimately, we will contribute an important foundational tool for flexible, creative, and impactful cloning in yeast. If successful, the centromeric plasmids will serve a multitude of purposes in the bioengineering and industrial fields. | |
| − | + | ||
</div> | </div> | ||
| Line 93: | Line 93: | ||
padding-top: 50px; | padding-top: 50px; | ||
padding-bottom: 50px; | padding-bottom: 50px; | ||
| + | padding-left: 30px; | ||
| + | padding-right: 30px; | ||
/*border-style: double; | /*border-style: double; | ||
border-color: white; | border-color: white; | ||
| Line 113: | Line 115: | ||
| − | + | .paragraph{ | |
font-size: 40px; | font-size: 40px; | ||
margin-right: 30px; | margin-right: 30px; | ||
| Line 120: | Line 122: | ||
</style> | </style> | ||
| + | |||
Revision as of 09:47, 20 October 2017
Before you start:
Please read the following pages:
Styling your wiki
You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.
While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.
Wiki template information
We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!
Editing your wiki
On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!
Use WikiTools - Edit in the black menu bar to edit this page
Tips
This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:
- State your accomplishments! Tell people what you have achieved from the start.
- Be clear about what you are doing and how you plan to do this.
- You have a global audience! Consider the different backgrounds that your users come from.
- Make sure information is easy to find; nothing should be more than 3 clicks away.
- Avoid using very small fonts and low contrast colors; information should be easy to read.
- Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2017 calendar
- Have lots of fun!
Inspiration
You can also view other team wikis for inspiration! Here are some examples:
Uploading pictures and files
You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to T--YourOfficialTeamName--NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)
UPLOAD FILES
