Line 15: | Line 15: | ||
= 1. Hardware = | = 1. Hardware = | ||
− | == 1. | + | == 1. Mircofluicdcs == |
+ | |||
== 2. Design for Light == | == 2. Design for Light == | ||
− | |||
− | We need 395nm light to activate GEM-GECO by Lumencor LED | + | === 2.1 Design for Light === |
+ | |||
+ | We need 395nm light to activate Calcium sensory protein GEM-GECO by Lumencor LED Illuminator. Then both red light and blue light activate CoChR or Chrimson by LCD projector. LCD projector and LED light source are merged by a double LH Adapter, which uses a semi-transparent mirror. Tee connect to microscopy light entrance. So we can use these two light together. | ||
{{SUSTech_Image_Center_8 | filename=T--SUSTech_Shenzhen--IMG_20170320_233120_HDR.jpg|width=800px|caption=<B>Fig. 2 Light Pathway of projecter and microscope</B> }} | {{SUSTech_Image_Center_8 | filename=T--SUSTech_Shenzhen--IMG_20170320_233120_HDR.jpg|width=800px|caption=<B>Fig. 2 Light Pathway of projecter and microscope</B> }} |
Revision as of 11:42, 22 October 2017
Hardware
Project
Contents
1. Hardware
1. Mircofluicdcs
2. Design for Light
2.1 Design for Light
We need 395nm light to activate Calcium sensory protein GEM-GECO by Lumencor LED Illuminator. Then both red light and blue light activate CoChR or Chrimson by LCD projector. LCD projector and LED light source are merged by a double LH Adapter, which uses a semi-transparent mirror. Tee connect to microscopy light entrance. So we can use these two light together.
Because the focus distance of origin lens in projector too short for microscopy, a longer focus distance lens is replaced. We install a new lens in projector.
To purify light form projector, origin blue channel filter is replaced in Chroma ET480/20X and red channel filter is replaced in Chroma ET630/20X. Dimirror is 89402bs, and emssion filter is 89402m.
References