Difference between revisions of "Team:Manchester/Improve"

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<p> The Manchester iGEM 2017 team have improved part <a href="http://partsregistry.org/Part:BBa_K562001"><b>BBa_K562001</b></a>, originally submitted by team <a href="https://2011.igem.org/Team:Dundee"></b>Dundee 2011</b></a>.</p>
 
<p> The Manchester iGEM 2017 team have improved part <a href="http://partsregistry.org/Part:BBa_K562001"><b>BBa_K562001</b></a>, originally submitted by team <a href="https://2011.igem.org/Team:Dundee"></b>Dundee 2011</b></a>.</p>
  
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We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible.  
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<p>We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible. <p/>
 
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We have also further improved this part by adding different Anderson promoters (<a href="http://parts.igem.org/Part:BBa_J23105">Low BBa_J23105<a/>, <a href="http://parts.igem.org/Part:BBa_J23108">Medium BBa_J23108<a/>, <a href="http://parts.igem.org/Part:BBa_J23104">High BBa_J23104<a/>) to control the expression level of the localisation tag. This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.  
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<p>We have also further improved this part by adding different Anderson promoters (<a href="http://parts.igem.org/Part:BBa_J23105">Low BBa_J23105<a/>, <a href="http://parts.igem.org/Part:BBa_J23108">Medium BBa_J23108<a/>, <a href="http://parts.igem.org/Part:BBa_J23104">High BBa_J23104<a/>) to control the expression level of the localisation tag. This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation. <p/>
 
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Revision as of 19:51, 22 October 2017