Acknowledgement
This project wouldn't be possible without commitment and dedication from many parties. It is our greatest pleasure to acknowledge the assistance and contribution of the individuals and groups who have helped us towards the successful completion of the project.
Adam Hannaford
Lab work on tags and their characterisation. Design of Experiment for protein expression optimisation.
Alice Fraser
Interlab study. Lab work on Eut proteins for their characterisation.
Amber Hall
Graphic and video editing, slide making and microscopy. Lab work. Wiki illustrations.
James Engleback
Lab work on characterisation of PPKs. Design of Experiment for protein expression optimisation.
Jessica Burns
Cloning of PPK and Eut parts into submission and expression vectors. Lab work on Eut proteins for their characterisation.
Maciej Słowiński
Seeking sponsors and looking for collaborations. Building business model.
Ong Jun Yang
Wiki development and phosphate starvation operon modelling. Lab work. 3D animations.
Owen Julianto Jonathan
Every bit of human practices and making cost estimation from continuous culture modelling. Lab work. Wiki illustrations.
Theodore Glenaldo
Interlab study and lab work. Safety forms submission. Wiki illustrations.
Research Institute
Manchester Institute of Biotechnology (MIB)
Project Advisors
Eriko Takano, Rainer Breitling
Advice on everything concerning the project.
Project Supervisors
William Finnigan, Marc Biarnes-Carrera, Ross Kent, Nicholas Weise, Jonathan Wilkes, Adam Amara
Advice on experimental design, troubleshooting, part assembly and characterisation, orders and deliveries, Design of Experiment, opportunities for outreach and articles.
Team Members
Adam Hannaford, Alice Fraser, Amber Hall, James Engleback, Jessica Burns, Maciej Słowiński, Ong Jun Yang, Owen Julianto Jonathan, Theodore Glenaldo
Trainings
Tanya Aspinall - Health & Safety induction
Sandra Taylor - Microscopy training
John Hughes - Ultracentrifugation training
Finance
Louise Barnes, Lisa Beattie
Human Practices
Dr. Andrew Balmer, Dr. Robert Meckin - on social implications of synthetic biology
Dr. Duncan Thomas - Manchester Institute of Innovation
Computational Modelling
Areti Tsigkinopoulou, Aliah Hawari
Advice on computational modelling of phosphate starvation operon, sources of scripts and papers on ensemble modelling.
Fundamentals
Martin Warren - Original paper on microcompartment-directed polyphosphate accumulation
Ashley Chessher - Eut plasmids and microscopy image samples
Inspiring previous work by HKU iGEM 2013, Dundee iGEM 2011, York iGEM 2015
Human Practices
Professor Issy Caffoor - Ex-Innovation Manager at Yorkshire Water
Jonathan Abra - Knowledge Transfer Network
Dr. Ben Reeve - CustoMem
Dr. Benjamin Tam - Isle Utilities
Dr. David Tompkins and PhD student Katie Ward - Aqua Enviro
Sarah Lyons - Davyhulme Treatment Works
Dr. Rick Watson - University of Manchester Intellectual Property (UMIP)
Dr. Linda Kahl - BioBricks Foundation
Department of Environment, Food and Rural Affairs (DEFRA)
Suppliers/ Sponsors
New England Biolabs, Qiagen, Promega, IDT, BioCatNet, IBCarb, Snapgene, Mathworks, Agilent
The University of Manchester, Manchester Metropolitan University
Design of Experiment (DoE)
Dr Markus Gershater - Synthace Ltd
Software
CellProfiller: pipeline used in the paper "Evidence for Improved Encapsulated Pathway Behavior in a Bacterial Microcompartment through Shell Protein Engineering" published in ACS Synthetic Biology
by Chuchu Zhang, Christopher Jakobson, Marilyn Slininger Lee and Danielle Tullman-Ercek