Team:Manchester/Basic Part
Basic Parts
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Please visit our Part Collection page for ALL PARTS.
| Part Name | BioBrick | Description | Part Type |
|---|---|---|---|
| LacUV5_EutS | BBa_K2213000 | The ethanolamine utilisation bacterial microcompartment (BMC) protein, EutS (from E. coli) under control of the LacUV5 inducible promoter. Contains RBS, terminators and all inducible Lac-system parts. EutS is tagged with His6. | Basic |
| TetR_EutMN | BBa_K2213001 | Ethanolamine Utilisation (Eut) bacterial micro-compartment (BMC) proteins EutM and EutN from E. coli, placed under the inducible Tetracycline promoter. Contains RBS, terminators and all tetp components. EutM is tagged with GFP and His6. EutN is tagged with FLAG. | Basic |
| araBAD_EutLK | BBa_K2213002 | The ethanolamine utilisation bacterial microcompartment (BMC) protein, EutLK (from E. coli) under control of the arabinose inducible promoter. Contains RBS, terminators and all tetp components. EutL is tagged with His6. EutK is tagged with FLAG. | Basic |
| cgPPK2_His6 | BBa_K2213003 | Corynebacterium PPK2 with a C terminal polyhistidine tag, and the PduD(1-20) localisation tag at the N terminal. Contains RBS and terminator. | Basic |
| PduD(1-20)_cgPPK2_mCherry_His6 | BBa_K2213004 | Corynebacterium PPK2 with a C terminal mCherry-polyhistidine tag, and the PduD(1-20) localisation tag at the N terminal. Contains RBS and terminator. | Basic |
| PduD(1-20)_mCherry_cgPPK2_His6 | BBa_K2213005 | Corynebacterium PPK2 with a C terminal polyhistidine tag, and the PduD(1-20) localisation tag and mCherry at the N terminal. Contains RBS and terminator. | Basic |
| LowPromoter_PduD(1-20)_mCherry | BBa_K2213006 | BBa_K562001,with the illegal XbaI site removed, and with a low promoter (BBa_J23105), and mCherry added. | Basic - Improved |
| MediumPromoter_PduD(1-20)_mCherry | BBa_K2213007 | BBa_K562001, with the illegal XbaI site removed, and with a medium promoter (BBa_J23108), and mCherry added. | Basic - Improved |
| HighPromoter_PduD(1-20)_mCherry | BBa_K2213008 | BBa_K562001, with the illegal XbaI site removed, and with a high promoter (BBa_J23104), and mCherry added. | Basic - Improved |
| LowPromoter_PduD(1-20)_mCherry_cgPPK2 | BBa_K2213009 | BBa_K2213006 with cgPPK2 added to the C terminus. | Basic |
