Difference between revisions of "Team:Manchester/Improve"

We have also further improved this part by adding different Anderson promoters (Low BBa_J23105, Medium BBa_J23108, High BBa_J23104) to control the expression level of the localisation tag. This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.

Part Name	BioBrick	Description	Part Type
LowPromoter_PduD(1-20)_mCherry	BBa_K2213006	Part Description	Basic
MediumPromoter_PduD(1-20)_mCherry	BBa_K2213007	Part Description.	Basic
HighPromoter_PduD(1-20)_mCherry	BBa_K2213008	Part Description.	Basic

@@ Line 70: / Line 70: @@
 <p>We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible. </p>
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-<p>We have also further improved this part by adding different Anderson promoters (<a href="http://parts.igem.org/Part:BBa_J23105">Low BBa_J23105<a/>, <a href="http://parts.igem.org/Part:BBa_J23108">Medium BBa_J23108<a/>, <a href="http://parts.igem.org/Part:BBa_J23104">High BBa_J23104<a/>) to control the expression level of the localisation tag. This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation. </p>
+<p>We have also further improved this part by adding different Anderson promoters (<a href="http://parts.igem.org/Part:BBa_J23105">Low BBa_J23105</a>, <a href="http://parts.igem.org/Part:BBa_J23108">Medium BBa_J23108</a>, <a href="http://parts.igem.org/Part:BBa_J23104">High BBa_J23104</a>) to control the expression level of the localisation tag. This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation. </p>