1. Hardware

1. Mircofluicdcs

2. Design for Light in spatio-temportal

Multiple devices of optics are designed and created for the various reqiument of experiments. All devices are attempt to modulate the spatio-temporal pattern elegantly and effective.

2.1 Device one: Arduio modulate Mercury lamp

A simple and effective device to

== 2.2 Device two:

2.1 Basic of mechanical and optics

We need 395nm light to activate Calcium indicator protein GEM-GECO by Lumencor LED Illuminator. Then both red light and blue light activate CoChR or Chrimson by LCD projector. LCD projector and LED Illuminator are merged by a double LH Adapter contained a semi-transparent mirror. Double LH Adapter connect to entrance of light in microscope. So we can use these two light source together.

Fig. 2Light Pathway of projecter and microscope

We choose projector as red and blue light source, because we can activate single cell or C. elegans with adjustable color and strength.

Fig. 3 Red, blue, green out from projecter

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Because the focus distance of origin lens in projector too short for microscopy, a longer focus distance lens is replaced. We install a new lens in projector.

Fig 5. Filter and mirrors in our system

To purify light form projector, origin blue channel filter is replaced in Chroma ET480/20X and red channel filter is replaced in Chroma ET630/20X. Dimirror is 89402bs, and emssion filter is 89402m.

=== 2.2 Time

References