Difference between revisions of "Team:SUSTech Shenzhen/Hardware"
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|size=70px|title=Hardware|subtitle=Create for wisdom of Life}}<!--------icon在page-header的utl参数里设置，参数size是其大小-------------> | |size=70px|title=Hardware|subtitle=Create for wisdom of Life}}<!--------icon在page-header的utl参数里设置，参数size是其大小-------------> | ||
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= 1. Hardware = | = 1. Hardware = | ||
| + | {{SUSTech_Image | filename=T--SUSTech_Shenzhen--Microfluicds&Microscope.jpg | caption=<B>Fig.1 Microfluidics chip couple with projector light source}} | ||
== 1. Mircofluicdcs == | == 1. Mircofluicdcs == | ||
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== 2. Design for Light in spatio-temportal == | == 2. Design for Light in spatio-temportal == | ||
| − | Multiple devices of optics are designed and created for the various reqiument of experiments. All devices are attempt to modulate the spatio-temporal pattern elegantly and | + | Multiple devices of optics are designed and created for the various reqiument of experiments. All devices are attempt to modulate the spatio-temporal pattern elegantly and effectively. |
| − | == 2.1 Device | + | |
| − | A simple and effective device to | + | |
| + | == 2.1 Device One: Arduino modulate Mercury lamp == | ||
| + | A simple and effective device to output pulse of certain wavelength of light. On time and off time of pusle is custom by Arduio. Wavelength of light is modified by replacing filter before beam expander. | ||
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| + | {{SUSTech_Image | filename=T--SUSTech_Shenzhen--MercuryLamp.jpg | caption=<B>Fig.2 (A) Arduino transfer the pusle command to servo. (B)The interview of MercuryLamp. (C)The servo fix at the original bottom. (D) The beam expander connect to steroscope}} | ||
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| + | == 2.2 Device Two: Prjoctor Tracker == | ||
| − | === 2.1 | + | ==== 2.2.1 Basical mechanics and optics= === |
| − | We need 395nm light to activate Calcium indicator protein GEM-GECO by Lumencor LED Illuminator. Then both red light and blue light activate CoChR or Chrimson by LCD projector. LCD projector and LED Illuminator are merged by a double LH Adapter contained a semi-transparent mirror. Double LH Adapter connect to entrance of light in microscope. So we can use these two light source together. | + | We need 395nm light to activate Calcium indicator protein GEM-GECO by Lumencor LED Illuminator. Then both red light and blue light activate CoChR or Chrimson by LCD projector. LCD projector and LED Illuminator are merged by a double LH Adapter contained a semi-transparent mirror. Double LH Adapter connect to entrance of light in microscope. So we can use these two light source together. |
| − | {{SUSTech_Image_Center_8 | filename=T--SUSTech_Shenzhen--IMG_20170320_233120_HDR.jpg|width=800px|caption=<B>Fig. | + | {{SUSTech_Image_Center_8 | filename=T--SUSTech_Shenzhen--IMG_20170320_233120_HDR.jpg|width=800px|caption=<B>Fig. 3</B> The apparatus connect microscope and projector. }} |
We choose projector as red and blue light source, because we can activate single cell or C. elegans with adjustable color and strength. | We choose projector as red and blue light source, because we can activate single cell or C. elegans with adjustable color and strength. | ||
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To purify light form projector, origin blue channel filter is replaced in [https://www.chroma.com/products/parts/et480-20x Chroma ET480/20X] and red channel filter is replaced in [https://www.chroma.com/products/parts/et630-20x Chroma ET630/20X]. Dimirror is [https://www.chroma.com/products/parts/89402bs 89402bs], and emssion filter is [https://www.chroma.com/products/parts/89402m 89402m]. | To purify light form projector, origin blue channel filter is replaced in [https://www.chroma.com/products/parts/et480-20x Chroma ET480/20X] and red channel filter is replaced in [https://www.chroma.com/products/parts/et630-20x Chroma ET630/20X]. Dimirror is [https://www.chroma.com/products/parts/89402bs 89402bs], and emssion filter is [https://www.chroma.com/products/parts/89402m 89402m]. | ||
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= References = | = References = | ||
Revision as of 16:31, 23 October 2017
Hardware
Create for wisdom of Life
Contents
1. Hardware
1. Mircofluicdcs
2. Design for Light in spatio-temportal
Multiple devices of optics are designed and created for the various reqiument of experiments. All devices are attempt to modulate the spatio-temporal pattern elegantly and effectively.
2.1 Device One: Arduino modulate Mercury lamp
A simple and effective device to output pulse of certain wavelength of light. On time and off time of pusle is custom by Arduio. Wavelength of light is modified by replacing filter before beam expander.
2.2 Device Two: Prjoctor Tracker
= 2.2.1 Basical mechanics and optics=
We need 395nm light to activate Calcium indicator protein GEM-GECO by Lumencor LED Illuminator. Then both red light and blue light activate CoChR or Chrimson by LCD projector. LCD projector and LED Illuminator are merged by a double LH Adapter contained a semi-transparent mirror. Double LH Adapter connect to entrance of light in microscope. So we can use these two light source together.
We choose projector as red and blue light source, because we can activate single cell or C. elegans with adjustable color and strength.
Because the focus distance of origin lens in projector too short for microscopy, a longer focus distance lens is replaced. We install a new lens in projector.
To purify light form projector, origin blue channel filter is replaced in Chroma ET480/20X and red channel filter is replaced in Chroma ET630/20X. Dimirror is 89402bs, and emssion filter is 89402m.
References
