Difference between revisions of "Team:SUSTech Shenzhen/Hardware"

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We need 395nm light to activate Calcium indicator protein GEM-GECO by Lumencor LED Illuminator. Then both red light and blue light activate CoChR or Chrimson by LCD projector. LCD projector and LED Illuminator are merged by a double LH Adapter contained a semi-transparent mirror. Double LH Adapter connect to entrance of light in microscope. So we can use these two light source together.
 
We need 395nm light to activate Calcium indicator protein GEM-GECO by Lumencor LED Illuminator. Then both red light and blue light activate CoChR or Chrimson by LCD projector. LCD projector and LED Illuminator are merged by a double LH Adapter contained a semi-transparent mirror. Double LH Adapter connect to entrance of light in microscope. So we can use these two light source together.
  
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{{SUSTech_Image_Center_8 | filename=T--SUSTech_Shenzhen--IMG_20170320_233120_HDR.jpg|width=900px|caption= Fig. 2Light Pathway of projecter and microscope }}
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{{SUSTech_Image_Center_8 | filename=T--SUSTech_Shenzhen--IMG_20170320_233120_HDR.jpg|width=800px|caption= Fig. 2 Light Pathway of projector and microscope }}
  
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We choose projector as red and blue light source, because we can activate single cell or //C. elegans// with adjustable color and strength.
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We choose projector as red and blue light source, because we can activate single cell or //C. elegans// with adjustable color and intensity. Because the focus distance of origin lens in projector too short for our microscopy(Nikon Ti-E), a longer focus distance lens is replaced. We install a new lens in projector. To purify light of the projector, origin blue channel filter is replaced in [https://www.chroma.com/products/parts/et480-20x Chroma ET480/20X] and red channel filter is replaced in [https://www.chroma.com/products/parts/et630-20x Chroma ET630/20X].  Dimirror is  [https://www.chroma.com/products/parts/89402bs 89402bs], and emssion filter is [https://www.chroma.com/products/parts/89402m 89402m].
  
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{{SUSTech_Image_Center_8 | filename=T--SUSTech_Shenzhen--RGBofProjecter.jpg| width=800px|caption=<B>Fig. 3 Red, blue, green out from projecter</B> }}
 
  
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{{SUSTech_Image_Center_8 | filename=T--SUSTech_Shenzhen--DSCF1972.JPG |width=800px| captionn=<B>Fig 4. Components of projecter }}
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{{SUSTech_Image_Center_8 | filename=T--SUSTech_Shenzhen--RGBofProjecter.jpg| width=800px|caption= Fig. 3 Red, blue, green out from projector}}
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{{SUSTech_Image_Center_8 | filename=T--SUSTech_Shenzhen--DSCF1972.JPG |width=800px| caption= Fig 4. Components of projector }}
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<相片墙：　投影仪拆机图，分光镜效果，新装上去的滤镜，>
  
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Because the focus distance of origin lens in projector too short for microscopy, a longer focus distance lens is replaced. We install a new lens in projector.
 
  
 
{{SUSTech_Image_Center_8 | filename=T--SUSTech_Shenzhen--SetofFilter.png | width=800px | caption=<B>Fig 5. Filter and mirrors in our system</B>}}
 
{{SUSTech_Image_Center_8 | filename=T--SUSTech_Shenzhen--SetofFilter.png | width=800px | caption=<B>Fig 5. Filter and mirrors in our system</B>}}
  
  
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To purify light form projector, origin blue channel filter is replaced in [https://www.chroma.com/products/parts/et480-20x Chroma ET480/20X] and red channel filter is replaced in [https://www.chroma.com/products/parts/et630-20x Chroma ET630/20X].  Dimirror is  [https://www.chroma.com/products/parts/89402bs 89402bs], and emssion filter is [https://www.chroma.com/products/parts/89402m 89402m].
 
  
 
=== 2.2 Time
 
=== 2.2 Time

Revision as of 16:16, 24 October 2017

Team SUSTC-Shenzhen



Hardware

Maker for wisdom of Life


1. Mircofluicdcs

Under construction


2. Design for Light in spatio-temporal

2.1 Device One: Arduino modulate Mercury lamp

A simple and effective device to output pulse of certain wavelength of light. On time and off time of pulse is custom by Arduio. Wavelength of light is modified by replacing filter before beam expander.


T--SUSTech Shenzhen--DSCF2143.jpg
Fig.2 (A) Arduino transfer the pusle command to servo. (B)The interview of MercuryLamp.　(C)The servo fix at the original bottom. (D) The beam expander connect to steroscope


T--SUSTech Shenzhen--lampmovent.gif
Fig. 3 Demo


2.2 Device Two: Projector Tracker

2.1 Basic mechanical and optics

We need 395nm light to activate Calcium indicator protein GEM-GECO by Lumencor LED Illuminator. Then both red light and blue light activate CoChR or Chrimson by LCD projector. LCD projector and LED Illuminator are merged by a double LH Adapter contained a semi-transparent mirror. Double LH Adapter connect to entrance of light in microscope. So we can use these two light source together.


T--SUSTech Shenzhen--IMG 20170320 233120 HDR.jpg
Fig. 2 Light Pathway of projector and microscope

We choose projector as red and blue light source, because we can activate single cell or //C. elegans// with adjustable color and intensity. Because the focus distance of origin lens in projector too short for our microscopy(Nikon Ti-E), a longer focus distance lens is replaced. We install a new lens in projector. To purify light of the projector, origin blue channel filter is replaced in Chroma ET480/20X and red channel filter is replaced in Chroma ET630/20X. Dimirror is 89402bs, and emssion filter is 89402m.


T--SUSTech Shenzhen--RGBofProjecter.jpg
Fig. 3 Red, blue, green out from projector


T--SUSTech Shenzhen--DSCF1972.JPG
Fig 4. Components of projector


<相片墙：　投影仪拆机图，分光镜效果，新装上去的滤镜，>


T--SUSTech Shenzhen--SetofFilter.png
Fig 5. Filter and mirrors in our system


=== 2.2 Time

References

Made by from the elegans.Inc in SUSTech_Shenzhen.

Licensed under CC BY 4.0.

  • ©2017 SUSTech_Shenzhen