Difference between revisions of "Team:Lethbridge/Results"
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| − | <div class="contentDiv" id="text30"><p class="segment2">Team is registered!</p> | + | <div class="contentDiv" id="text30"><p class="segment2">Team is registered! <br> <br>Project is showcased on wiki <br><br>Project attributions clearly detailed <br><br>Safety forms submitted <br><br>Judging form completed <br><br>Parts documented on the registry <br><br>X (list #) parts submitted <br><br>Participated in the InterLab study |
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| − | <div class="contentDiv" id="text30"><p class ="segment2">X parts characterized and documented</p> | + | <div class="contentDiv" id="text30"><p class ="segment2">X parts characterized and documented<br><br>Worked closely with user groups to inform our design<br><br>Assisted Lethbridge High School with wet lab work and received help with education interviews<br><br>Collaborated with Florida State University (add details) |
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| − | <div class="contentDiv" id="text30"><p class ="segment2">Provided simplified protocols and learning tools for the education system in collaboration with the Lethbridge High School Team</p> | + | <div class="contentDiv" id="text30"><p class ="segment2">Provided simplified protocols and learning tools for the education system in collaboration with the Lethbridge High School Team<br><br>Addressed policy issues regarding cell-free systems<br><br>Assessed the biosecurity implications of our project<br><br>Developed and tested software tools for biocontainment and to mitigate dual-use<br><br>Improved X parts by optimizing coding sequences for optimal expression in E. coli and for simple purification<br><br>Demonstrated proof of concept for multi-protein purification to simplify the system |
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Revision as of 23:55, 27 October 2017
Team is registered!
Project is showcased on wiki
Project attributions clearly detailed
Safety forms submitted
Judging form completed
Parts documented on the registry
X (list #) parts submitted
Participated in the InterLab study
X parts characterized and documented
Worked closely with user groups to inform our design
Assisted Lethbridge High School with wet lab work and received help with education interviews
Collaborated with Florida State University (add details)
Provided simplified protocols and learning tools for the education system in collaboration with the Lethbridge High School Team
Addressed policy issues regarding cell-free systems
Assessed the biosecurity implications of our project
Developed and tested software tools for biocontainment and to mitigate dual-use
Improved X parts by optimizing coding sequences for optimal expression in E. coli and for simple purification
Demonstrated proof of concept for multi-protein purification to simplify the system
TX-TL Proteins
Test Overexpressions
We conducted test overexpressions of our constructs in BL21 DE3 Gold, an E. coli strain capable of overexpressing T7 RNA polymerase by induction with IPTG.
Multi-Protein Purification
tRNA
tRNA Purification
Validation Construct
In vitro Transcription
To confirm our ability to detect successful transcription, the spinach aptamer was in vitro transcribed using T7 RNA polymerase (previously purified) and purified by phenol chloroform extraction. Following addition of the fluorophore, DFHBI, fluorescence was measured using a fluorimeter. The fluorimeter data illustrates that fluorescence was observed following addition of DFHBI, with an excitation of 447nm and emission of 497nm.
