Difference between revisions of "Team:Lethbridge/Results"

Line 34: Line 34:
 
#image50 {width:50; height: 100%; vertical-align:middle;}
 
#image50 {width:50; height: 100%; vertical-align:middle;}
 
#image40 {width:40; height: 100%; vertical-align:middle; margin-left: 10%; margin-right: 10%}
 
#image40 {width:40; height: 100%; vertical-align:middle; margin-left: 10%; margin-right: 10%}
 +
 +
/* RESULTS TABLE BEGINS */
 +
    #resultsTable {width: 702px; border: hidden !important;}
 +
    #resultsTable th {width: 234px;}
 +
    #resultsTable th img {}
 +
    #resultsTable tr {font-size: calc(14px + 0.5vw) !important; line-height: calc(1.1em + 0.5vw) !important; text-align: center !important; vertical-align: top;}
 +
    #resultsTable td {padding-top: 15px; width: 234px; border: hidden !important;}
 +
/* RESULTS TABLE ENDS */
  
 
</style>
 
</style>
Line 40: Line 48:
 
<div><img src="https://static.igem.org/mediawiki/2017/6/68/Banner_PRresults.png"  class="center fit"></div>
 
<div><img src="https://static.igem.org/mediawiki/2017/6/68/Banner_PRresults.png"  class="center fit"></div>
 
<br><br>
 
<br><br>
−
 
−
 
  
 
<center>
 
<center>
−
    <div class="segmentContainer">
+
<div>
−
        <div class="contentDiv" id="image40"><img src="https://static.igem.org/mediawiki/2017/9/9a/Lethbridge_Bronze_Trophy.png" width=128px; height=166.67px; padding:10px; class="testImage" p class="segment2"/>
+
<table id="resultsTable">
−
        </div>
+
    <th><img src="https://static.igem.org/mediawiki/2017/9/9a/Lethbridge_Bronze_Trophy.png" width=128px; height=166.67px></th>
−
        <div class="contentDiv" id="image40"><img src="https://static.igem.org/mediawiki/2017/8/83/Lethbridge_Silver_Trophy.png" width=128px; height=166.67px; padding:10px; class="testImage" p class="segment2"/>
+
    <th><img src="https://static.igem.org/mediawiki/2017/8/83/Lethbridge_Silver_Trophy.png" width=128px; height=166.67px></th>
−
        </div>
+
    <th><img src="https://static.igem.org/mediawiki/2017/d/dd/Lethbridge_Gold_Trophy.png" width=128px; height=166.67px></th>
−
        <div class="contentDiv" id="image40"><img src="https://static.igem.org/mediawiki/2017/d/dd/Lethbridge_Gold_Trophy.png" width=128px; height=166.67px; padding:10px; class="testImage" p class="segment2"/>
+
−
        </div>
+
−
    </div>
+
−
</center>
+
−
    <div style="clear: both"></div><br>
+
  
−
<center>
+
     <tr id="row1">
−
     <div class="segmentContainer">
+
         <td>Team is registered</td>
−
         <div class="contentDiv" id="text30"><p class="segment2">Team is registered! <br> <br>Project is showcased on wiki <br><br>Project attributions clearly detailed <br><br>Safety forms submitted <br><br>Judging form completed <br><br>Parts documented on the registry <br><br>X (list #) parts submitted <br><br>Participated in the InterLab study
+
        <td>X parts characterized and documented</td>
−
</p>
+
        <td>Provided simplified protocols and learning tools for the education system in collaboration with the Lethbridge High School Team</td>
−
        </div>
+
    </tr>
−
        <div class="contentDiv" id="text30"><p class ="segment2">X parts characterized and documented<br><br>Worked closely with user groups to inform our design<br><br>Assisted Lethbridge High School with wet lab work and received help with education interviews<br><br>Collaborated with Florida State University (add details)
+
   
−
</p>
+
    <tr id="row2">
−
        </div>
+
        <td>Project is showcased on wiki</td>
−
        <div class="contentDiv" id="text30"><p class ="segment2">Provided simplified protocols and learning tools for the education system in collaboration with the Lethbridge High School Team<br><br>Addressed policy issues regarding cell-free systems<br><br>Assessed the biosecurity implications of our project<br><br>Developed and tested software tools for biocontainment and to mitigate dual-use<br><br>Improved X parts by optimizing coding sequences for optimal expression in E. coli and for simple purification<br><br>Demonstrated proof of concept for multi-protein purification to simplify the system
+
        <td>Worked closely with user groups to inform our design</td>
−
</p>
+
        <td>Addressed policy issues regarding cell-free systems</td>
−
         </div>
+
    </tr>
−
     </div>
+
   
 +
    <tr id="row3">
 +
        <td>Project attributions clearly detailed </td>
 +
        <td>Assisted Lethbridge High School with wet lab work and received help with education interviews</td>
 +
        <td>Assessed the biosecurity implications of our project</td>
 +
    </tr>
 +
   
 +
    <tr id="row4">
 +
        <td>Safety forms submitted</td>
 +
        <td>Collaborated with Florida State University (add details)</td>
 +
        <td>Developed and tested software tools for biocontainment and to mitigate dual-use</td>
 +
    </tr>
 +
   
 +
    <tr id="row5">
 +
        <td>Judging form completed</td>
 +
        <td></td>
 +
        <td>Improved X parts by optimizing coding sequences for optimal expression in E. coli and for simple purification</td>
 +
    </tr>
 +
   
 +
    <tr id="row6">
 +
        <td>Parts documented on the registry</td>
 +
        <td></td>
 +
        <td>Demonstrated proof of concept for multi-protein purification to simplify the system</td>
 +
    </tr>
 +
   
 +
    <tr id="row7">
 +
         <td>X (list #) parts submitted</td>
 +
        <td></td>
 +
        <td></td>
 +
     </tr>
 +
   
 +
    <tr id="row8">
 +
        <td>Participated in the InterLab study</td>
 +
        <td></td>
 +
        <td></td>
 +
    </tr>
 +
    </table>
 +
</div>
 
</center>
 
</center>
−
 
−
  <div style="clear: both"></div>
 
  
 
<br><br><br>
 
<br><br><br>

Revision as of 18:42, 28 October 2017



Team is registered X parts characterized and documented Provided simplified protocols and learning tools for the education system in collaboration with the Lethbridge High School Team
Project is showcased on wiki Worked closely with user groups to inform our design Addressed policy issues regarding cell-free systems
Project attributions clearly detailed Assisted Lethbridge High School with wet lab work and received help with education interviews Assessed the biosecurity implications of our project
Safety forms submitted Collaborated with Florida State University (add details) Developed and tested software tools for biocontainment and to mitigate dual-use
Judging form completed Improved X parts by optimizing coding sequences for optimal expression in E. coli and for simple purification
Parts documented on the registry Demonstrated proof of concept for multi-protein purification to simplify the system
X (list #) parts submitted
Participated in the InterLab study



TX-TL Proteins

Test Overexpressions

We conducted test overexpressions of our constructs in BL21 DE3 Gold, an E. coli strain capable of overexpressing T7 RNA polymerase by induction with IPTG.

Multi-Protein Purification











tRNA

tRNA Purification











Validation Construct

In vitro Transcription

To confirm our ability to detect successful transcription, the spinach aptamer was in vitro transcribed using T7 RNA polymerase (previously purified) and purified by phenol chloroform extraction. Following addition of the fluorophore, DFHBI, fluorescence was measured using a fluorimeter. The fluorimeter data illustrates that fluorescence was observed following addition of DFHBI, with an excitation of 447nm and emission of 497nm.

PURExpress