Difference between revisions of "Team:Manchester/Improve"
Ojjonathan (Talk | contribs) |
Ojjonathan (Talk | contribs) |
||
| Line 166: | Line 166: | ||
<p style="text-align: center; font-size: 18px!important">We have also further improved this part by adding different Anderson promoters (<a href="http://parts.igem.org/Part:BBa_J23105">Low BBa_J23105</a>, <a href="http://parts.igem.org/Part:BBa_J23108">Medium BBa_J23108</a>, <a href="http://parts.igem.org/Part:BBa_J23104">High BBa_J23104</a>) to control the expression level of the localisation tag. This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation. </p> | <p style="text-align: center; font-size: 18px!important">We have also further improved this part by adding different Anderson promoters (<a href="http://parts.igem.org/Part:BBa_J23105">Low BBa_J23105</a>, <a href="http://parts.igem.org/Part:BBa_J23108">Medium BBa_J23108</a>, <a href="http://parts.igem.org/Part:BBa_J23104">High BBa_J23104</a>) to control the expression level of the localisation tag. This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation. </p> | ||
</div> | </div> | ||
| − | <br | + | <br> |
| − | + | ||
<div class="plan" style="background-color: #f5fcff!important"> | <div class="plan" style="background-color: #f5fcff!important"> | ||
Revision as of 20:28, 30 October 2017
Improved Part
Please visit our Part Collection page for ALL PARTS.
The Manchester iGEM 2017 team have improved part BBa_K562001, originally submitted by team Dundee 2011.
We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible.
We have also further improved this part by adding different Anderson promoters (Low BBa_J23105, Medium BBa_J23108, High BBa_J23104) to control the expression level of the localisation tag. This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.
| Part Name | BioBrick | Description | Part Type |
|---|---|---|---|
| LowPromoter_PduD(1-20)_mCherry | BBa_K2213006 | BBa_K562001 with the illegal XbaI site removed, and with a low promoter (BBa_J23105), and mCherry added. | Basic - Improved |
| MediumPromoter_PduD(1-20)_mCherry | BBa_K2213007 | BBa_K562001 with the illegal XbaI site removed, and with a medium promoter (BBa_J23108), and mCherry added. | Basic - Improved |
| HighPromoter_PduD(1-20)_mCherry | BBa_K2213008 | BBa_K562001 with the illegal XbaI site removed, and with a high promoter (BBa_J23104), and mCherry added. | Basic - Improved |
