Difference between revisions of "Team:Manchester/Improve"
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<td style="border:1px black solid;" class="break">LowPromoter_PduD(1-20)_mCherry</td> | <td style="border:1px black solid;" class="break">LowPromoter_PduD(1-20)_mCherry</td> | ||
<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213006" target="_blank"><b>BBa_K2213006</b></a></td> | <td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213006" target="_blank"><b>BBa_K2213006</b></a></td> | ||
| − | <td style="border:1px black solid;">BBa_K562001 with the illegal XbaI site removed, and with a low promoter (BBa_J23105), and mCherry added.</td> | + | <td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K562001" target="_blank"><b>BBa_K562001</b></a>,with the illegal XbaI site removed, and with a low promoter (<a href="http://parts.igem.org/Part:BBa_J23105" target="_blank"><b>BBa_J23105</b></a>), and mCherry added.</td> |
<td style="border:1px black solid;">Basic - Improved</td> | <td style="border:1px black solid;">Basic - Improved</td> | ||
</tr> | </tr> | ||
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<td style="border:1px black solid;" class="break">MediumPromoter_PduD(1-20)_mCherry</td> | <td style="border:1px black solid;" class="break">MediumPromoter_PduD(1-20)_mCherry</td> | ||
<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213007"target="_blank"><b>BBa_K2213007</b></a></td> | <td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213007"target="_blank"><b>BBa_K2213007</b></a></td> | ||
| − | <td style="border:1px black solid;">BBa_K562001 with the illegal XbaI site removed, and with a medium promoter (BBa_J23108), and mCherry added.</td> | + | <td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K562001" target="_blank"><b>BBa_K562001</b></a>, with the illegal XbaI site removed, and with a medium promoter (<a href="http://parts.igem.org/Part:BBa_J23108" target="_blank"><b>BBa_J23108</b></a>), and mCherry added.</td> |
<td style="border:1px black solid;">Basic - Improved</td> | <td style="border:1px black solid;">Basic - Improved</td> | ||
</tr> | </tr> | ||
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<td style="border:1px black solid;" class="break">HighPromoter_PduD(1-20)_mCherry</td> | <td style="border:1px black solid;" class="break">HighPromoter_PduD(1-20)_mCherry</td> | ||
<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213008"target="_blank"><b>BBa_K2213008</b></a></td> | <td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213008"target="_blank"><b>BBa_K2213008</b></a></td> | ||
| − | <td style="border:1px black solid;">BBa_K562001 with the illegal XbaI site removed, and with a high promoter (BBa_J23104), and mCherry added.</td> | + | <td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K562001" target="_blank"><b>BBa_K562001</b></a>, with the illegal XbaI site removed, and with a high promoter (<a href="http://parts.igem.org/Part:BBa_J23104" target="_blank"><b>BBa_J23104</b></a>), and mCherry added.</td> |
<td style="border:1px black solid;">Basic - Improved</td> | <td style="border:1px black solid;">Basic - Improved</td> | ||
| − | |||
</table> | </table> | ||
Revision as of 17:19, 31 October 2017
Improved Part
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Please visit our Part Collection page for ALL PARTS.
The Manchester iGEM 2017 team have improved part BBa_K562001, originally submitted by team Dundee 2011.
We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible.
We have also further improved this part by adding different Anderson promoters (Low BBa_J23105, Medium BBa_J23108, High BBa_J23104) to control the expression level of the localisation tag.
This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.
| Part Name | BioBrick | Description | Part Type |
|---|---|---|---|
| LowPromoter_PduD(1-20)_mCherry | BBa_K2213006 | BBa_K562001,with the illegal XbaI site removed, and with a low promoter (BBa_J23105), and mCherry added. | Basic - Improved |
| MediumPromoter_PduD(1-20)_mCherry | BBa_K2213007 | BBa_K562001, with the illegal XbaI site removed, and with a medium promoter (BBa_J23108), and mCherry added. | Basic - Improved |
| HighPromoter_PduD(1-20)_mCherry | BBa_K2213008 | BBa_K562001, with the illegal XbaI site removed, and with a high promoter (BBa_J23104), and mCherry added. | Basic - Improved |
