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Revision as of 00:49, 1 November 2017



Logbook

I know what you did last summer! KU Leuven iGEM Team 2017 has gone through some ups and downs, but that didn't stop them from being creative and to explore the world of science. Other than that, a lot of fun activities were planned. To know more about their adventure, feel free to go down the memory lane with us.




  • 21 - 27 Feb

    First Meeting


    This is the meeting where the team spirit was established and missing pieces of our adventure to success were collected. For example, who is going to be our PI? How do we get sponsors? Who will be our leader to guide us all the way through to the end of the project? Every team member was assigned a subcategory, which they will complete with passion and dedication. A very subtle move towards possible project ideas was made during this meeting.

  • 27 Feb - 7 Mar

    Project Ideas


    This week, we discussed the many proposals about project ideas along with some professors. There were some remarkable ideas on the table: Development of an ecosystem in a space station, DNA fitting to predict an individual’s health, weight and nutrition, introduction of a filter over intestinal villus for better uptake of gluten in Coeliac patients, and more.

    We also completed our team registration and team leaders were chosen for the subcategories. Nothing could stop us from entering this!

  • 7 - 13 Mar

    Brainstorming


    The voting deadline for project ideas was nearing. In the meeting, a lot of brainstorming happened and a lot of new ideas were added to the list. The layout for a sponsor brochure was also created. We reminisced about our childhood and our first contact with science for inspiration on how we might educate the local children. During the meeting, one of the students who is an avid traveler booked flight tickets.



    Boston, HERE WE COME!

  • 14 - 21 Mar

    A visit from two familiar figures

    This week, we had a very special meeting. Two ex-iGEM members came over and gave us advice about their struggles and experiences in their iGEM team in 2015. A lot of stories were shared and the trust of KU Leuven was handed over to the current iGEM team as every member got an account and joined the KU_Leuven team on igem.org. There is also a rumor going around, which tells us that six professors are going to visit us within two weeks!

  • 21 - 27 Mar

    Sponsor Brochure


    The first activity of the iGEM Team 2017 was planned, which is having dinner together in the following week. This week,an electrophysiology professor came to visit the KUL iGEM Team, which is a great honor. A lot of ideas were exchanged and it gave us a better insight into our potential project. The requirements for multiple projects are being checked and the final touches to the sponsor brochure was done. Sponsors were reaching out to the KUL iGEM Team and mutually beneficial agreements have been made.

  • 28 Mar - 17 Apr

    3 Projects and 3 Teams

    This week, which is also the last week before spring break, the team was divided into three groups, each working on a potential project. Multiple methods and perspectives were discussed, pushing our creativity to the limit. We planned two meetings with potential sponsors which wrapped up the first part of preparations before spring break hit in.

  • 18 Apr

    Arrival of the DNA kit

    some text
    While it is still half a year apart from the Giant Jamboree, the team was already been looking for accommodations in Boston. Visas were applied for, rooms were booked and our summer schedule slowly materialized much like the sunshine in Belgium. As our final chosen project slowly got its shape, students explored their inner creativity and set up a potential draft for their logo. Our DNA distribution kit from iGEM has arrived and we were so excited!

  • 29 Apr

    KUL iGEM goes VIB

    The KULeuven iGEM Team 2017 got the opportunity to spread a word about iGEM on the VIB conference with over 700 attendees! Two courageous teammates did a presentation and it was a nerve-wracking day for them. The KULeuven Team was there to support them and they nailed it! Congratulations and thank you to Pieter-Jan and Jana for the great presentation!

  • 10 - 26 June

    iGEM vs Exams

    Despite the fact that the students are in the midst of the exams, impromptu meetings were planned to iron out the last details about the project. We set the summer schedule and chose a logo. We even squeezed in some fascinating scientific articles during our exam study breaks.

  • 27 June

    KUL iGEM goes Wageningen

    some text
    The KULeuven iGEM team got an invitation from the iGEM Wageningen team for the iGEM Benelux meet up! Teams from Groningen, Delft and Louvain joined in and the KU Leuven Team attended many interesting presentations and even went home with a package of knowledge, inspiration and new ideas. Thank you to Wageningen for having us and the team from Groningen for inspiring us with your 3D phage model!

  • 28 June

    Start of the Labwork


    All the members of the KU Leuven iGEM Team 2017 have received a short introduction about lab safety.

  • 29 June

    Stable mHCN-HEK cell line defreeze and introduction to cell splitting

    The iGEM summer has finally started! The students collected information and shared them with the teammates. Remarkable scientific articles have been discussed and the very first experiments were carried out.
    We got an introduction about how to handle eukaryotic cells. We learnt how to split cells, how to refresh the medium and so on.

  • 30 June

    Introduction to cell splitting

    We were taught on how to split HEK-cells and we practiced this.

  • 3 - 7 July

    Primer design, transformation and first Ca2+-imaging


    Krebs buffers with different K-concentrations: 5 mM, 55 mM and 155 mM.

  • 10 July

    Koffiekoeken!


    As we all know, Monday is the beginning of a great week, but also one of the hardest mornings to get out of bed. Luckily, Liesbeth, one of the teammates, is like a ray of sunshine. That morning she provided the team with fresh and tasty pastries, along with a pretty smile.

  • 12 July

    Unboxing


    Pieter-Jan, one of the students of the team, was completed buried under the boxes. We were stocked with a variety of equipment, thanks to our sponsors.

  • 11 July - 3 August

    Patch clamp introduction


    A few students from our team are learning the patch clamp technique. Their curiosity and excitement was written all over their faces as they discovered ion channels on their first day in the electrophysiology lab. Jana, Mathias and Evelien were being trained in Patch Clamping with TRPM3 cells which took from 11 July till the 3rd of August.

  • 20 – 24 July

    The failure of the PCR


    On the 20th of July, the first PCR reactions for mHCN, hERG, SHIR were done, but unfortunately there were no results. On the 21st, we repeated the PCRs. Now, only ‘hERG’-DNA was amplified.
    On the 24th, we got the same results as on the 21st.

  • 25 July – 5 August

    Purification, digestion, ligation, transformation of the ‘hERG’-DNA

    From the 25th of July till the 5th of August, we attempted to purify, digest, ligate and transform the ‘hERG’-DNA. Unfortunately, all these attempts failed.

  • 27 July - 7 August

    Ca2+ imaging with different K-concentrations