Difference between revisions of "Team:Manchester/Improve"

 
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<h2 class="border" style="margin-top: 5vh; text-align: center">Improved Part</h2>
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<p style="color: #f5fcff!important">f</p><br>
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<p style="text-align: center; font-size: 20px!important"><i> Please visit our <a href="https://2017.igem.org/Team:Manchester/Part_Collection"><b>Part Collection</b></a> page for ALL PARTS.</i></p>
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<p style="text-align: center; font-size: 18px!important"> The Manchester iGEM 2017 team have improved part <a href="http://parts.igem.org/Part:BBa_K562001"><b>BBa_K562001</b></a>, originally submitted by team <a href="https://2011.igem.org/Team:Dundee"></b>Dundee 2011</b></a>.</p>
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<p style="text-align: center; font-size: 18px!important">We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible. </p>
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<p style="text-align: center; font-size: 18px!important">We have also further improved this part by adding different Anderson promoters (<a href="http://parts.igem.org/Part:BBa_J23105">Low BBa_J23105</a>, <a href="http://parts.igem.org/Part:BBa_J23108">Medium BBa_J23108</a>, <a href="http://parts.igem.org/Part:BBa_J23104">High BBa_J23104</a>) to control the expression level of the localisation tag.</p>
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<p style="text-align: center; font-size: 18px!important">This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.</p>
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<th style="border:1px black solid;background: #d4f4fc;"><b>Part Name</b></th>
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                <th style="border:1px black solid;background: #d4f4fc;"><b>BioBrick</b></th>
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                <th style="border:1px black solid;background: #d4f4fc;"><b>Description</b></th>
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<td style="border:1px black solid;" class="break">LowPromoter_PduD(1-20)_mCherry</td>
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<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213006" target="_blank"><b>BBa_K2213006</b></a></td>
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K562001" target="_blank"><b>BBa_K562001</b></a>,with the illegal XbaI site removed, and with a low promoter (<a href="http://parts.igem.org/Part:BBa_J23105" target="_blank"><b>BBa_J23105</b></a>), and mCherry added.</td>
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<td style="border:1px black solid;">Basic - Improved</td>
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<td style="border:1px black solid;" class="break">MediumPromoter_PduD(1-20)_mCherry</td>
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<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213007"target="_blank"><b>BBa_K2213007</b></a></td>
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K562001" target="_blank"><b>BBa_K562001</b></a>, with the illegal XbaI site removed, and with a medium promoter (<a href="http://parts.igem.org/Part:BBa_J23108" target="_blank"><b>BBa_J23108</b></a>), and mCherry added.</td>
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<td style="border:1px black solid;">Basic - Improved</td>
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<td style="border:1px black solid;" class="break">HighPromoter_PduD(1-20)_mCherry</td>
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<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213008"target="_blank"><b>BBa_K2213008</b></a></td>
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K562001" target="_blank"><b>BBa_K562001</b></a>, with the illegal XbaI site removed, and with a high promoter (<a href="http://parts.igem.org/Part:BBa_J23104" target="_blank"><b>BBa_J23104</b></a>), and mCherry added.</td>
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<td style="border:1px black solid;">Basic - Improved</td>
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<p> The Manchester iGEM 2017 team have improved part <a href="http://partsregistry.org/Part:BBa_K562001"><b>BBa_K562001</b></a>, originally submitted by <a href="https://2011.igem.org/Team:Dundee"></b>Dundee 2011</b></a>.</p>
 
 
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<a class="floatleft1 project projectlink" href= " https://2017.igem.org/Team:Manchester/Part_Collection " ><img src="https://static.igem.org/mediawiki/2017/3/3c/T--Manchester--NEXTARROWPARTS17.jpg"></a>
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<a class="floatright1 project projectlink" href= " https://2017.igem.org/Team:Manchester/Composite_Part " ><img src="https://static.igem.org/mediawiki/2017/1/11/T--Manchester--BACKARROWPARTSMAN17.jpg"></a>
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Latest revision as of 21:07, 1 November 2017

HOME
TEAM
PROJECT
Overview
Description
Results
Demonstration
Medal Criteria
Achievement
NOTEBOOK
Protocols
Diary
Lab Book
InterLab
Measurement
PARTS
Parts
Basic Parts
Composite Parts
Improved Parts
Part Collection
HUMAN PRACTICES
Silver HP
Water Industry
Integrated and Gold
Intellectual Property
Public Engagement
Entrepreneurship
MODELLING
Overview
D. O. Experiment
Continuous Culture
PHO Operon
JUDGING FORM

Improved Part



f


Please visit our Part Collection page for ALL PARTS.


The Manchester iGEM 2017 team have improved part BBa_K562001, originally submitted by team Dundee 2011.

We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible.

We have also further improved this part by adding different Anderson promoters (Low BBa_J23105, Medium BBa_J23108, High BBa_J23104) to control the expression level of the localisation tag.

This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.


Part Name BioBrick Description Part Type
LowPromoter_PduD(1-20)_mCherry BBa_K2213006 BBa_K562001,with the illegal XbaI site removed, and with a low promoter (BBa_J23105), and mCherry added. Basic - Improved
MediumPromoter_PduD(1-20)_mCherry BBa_K2213007 BBa_K562001, with the illegal XbaI site removed, and with a medium promoter (BBa_J23108), and mCherry added. Basic - Improved
HighPromoter_PduD(1-20)_mCherry BBa_K2213008 BBa_K562001, with the illegal XbaI site removed, and with a high promoter (BBa_J23104), and mCherry added. Basic - Improved