Difference between revisions of "Team:Manchester/Improve"

 
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font: 18px black 'Calibri', sans-serif
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body {
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table {
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   font-family: 'Open Sans', sans-serif;
 
   font-family: 'Open Sans', sans-serif;
 
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</head>
 
</head>
 
<body>
 
<body>
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<div style="margin-top: 40px" class="clear"></div>
 
  
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<div class="clear"></div>
  
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<div class="clear" style="padding: 0px 6vw; border: 3px solid transparent">
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<div class="plan" style="background-color: #f5fcff!important">
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<div class="col-md-12 sectionfirst" style="background-color: #f5fcff!important">
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<h2 class="border" style="margin-top: 5vh; text-align: center">Improved Part</h2>
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</div>
  
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<center>
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<div class="plan" style="padding: 0px 18vw; background-color: #f5fcff!important">
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<h2 style="margin-top: 70px">
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<br><br>
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<font color"#000000">Improved Parts</font>
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<center>
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</h2>
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<p style="color: #f5fcff!important">f</p><br>
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<p style="text-align: center; font-size: 20px!important"><i> Please visit our <a href="https://2017.igem.org/Team:Manchester/Part_Collection"><b>Part Collection</b></a> page for ALL PARTS.</i></p>
 +
<br>
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<p style="text-align: center; font-size: 18px!important"> The Manchester iGEM 2017 team have improved part <a href="http://parts.igem.org/Part:BBa_K562001"><b>BBa_K562001</b></a>, originally submitted by team <a href="https://2011.igem.org/Team:Dundee"></b>Dundee 2011</b></a>.</p>
  
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<p><i> Please visit our <a href="https://2017.igem.org/Team:Manchester/Part_Collection"><b>Part Collection</b></a> page for ALL PARTS.</i></p>
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<p style="text-align: center; font-size: 18px!important">We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible. </p>
  
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<br></br>
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<p style="text-align: center; font-size: 18px!important">We have also further improved this part by adding different Anderson promoters (<a href="http://parts.igem.org/Part:BBa_J23105">Low BBa_J23105</a>, <a href="http://parts.igem.org/Part:BBa_J23108">Medium BBa_J23108</a>, <a href="http://parts.igem.org/Part:BBa_J23104">High BBa_J23104</a>) to control the expression level of the localisation tag.</p>
−
<p> The Manchester iGEM 2017 team have improved part <a href="http://partsregistry.org/Part:BBa_K562001"><b>BBa_K562001</b></a>, originally submitted by team <a href="https://2011.igem.org/Team:Dundee"></b>Dundee 2011</b></a>.</p>
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<p>Improvement over old part:
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<p style="text-align: center; font-size: 18px!important">This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.</p>
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<br/>
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<br>
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We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible.
+
</div>
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<br/>
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−
We have also further improved this part by adding different anderson promoters (BB LInk bb link...) to control the expression level of the localisation tag. This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.  
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</p>
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<table style="width: 1000px; max-width: 95vw">
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<table class="table table-bordered table-striped" style="width: 1000px; max-width: 95vw">
  
 
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     <col width="200">
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<tr>
 
<tr>
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<th style="border:1px black solid;background: #add8e6;"><b>Part Name</b></th>
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<th style="border:1px black solid;background: #d4f4fc;"><b>Part Name</b></th>
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                 <th style="border:1px black solid;background: #add8e6;"><b>BioBrick</b></th>
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                 <th style="border:1px black solid;background: #d4f4fc;"><b>BioBrick</b></th>
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                 <th style="border:1px black solid;background: #add8e6;"><b>Description</b></th>
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                 <th style="border:1px black solid;background: #d4f4fc;"><b>Description</b></th>
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<th style="border:1px black solid;background: #add8e6;"><b>Part Type</b></th>
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<th style="border:1px black solid;background: #d4f4fc;"><b>Part Type</b></th>
 
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<td style="border:1px black solid;" class="break">LowPromoter_PduD(1-20)_mCherry</td>
 
<td style="border:1px black solid;" class="break">LowPromoter_PduD(1-20)_mCherry</td>
 
<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213006" target="_blank"><b>BBa_K2213006</b></a></td>
 
<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213006" target="_blank"><b>BBa_K2213006</b></a></td>
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<td style="border:1px black solid;">Part Description</td>
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K562001" target="_blank"><b>BBa_K562001</b></a>,with the illegal XbaI site removed, and with a low promoter (<a href="http://parts.igem.org/Part:BBa_J23105" target="_blank"><b>BBa_J23105</b></a>), and mCherry added.</td>
−
<td style="border:1px black solid;">Basic</td>
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<td style="border:1px black solid;">Basic - Improved</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td style="border:1px black solid;" class="break">MediumPromoter_PduD(1-20)_mCherry</td>
 
<td style="border:1px black solid;" class="break">MediumPromoter_PduD(1-20)_mCherry</td>
 
<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213007"target="_blank"><b>BBa_K2213007</b></a></td>
 
<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213007"target="_blank"><b>BBa_K2213007</b></a></td>
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<td style="border:1px black solid;">Part Description.</td>
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K562001" target="_blank"><b>BBa_K562001</b></a>, with the illegal XbaI site removed, and with a medium promoter (<a href="http://parts.igem.org/Part:BBa_J23108" target="_blank"><b>BBa_J23108</b></a>), and mCherry added.</td>
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<td style="border:1px black solid;">Basic</td>
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<td style="border:1px black solid;">Basic - Improved</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td style="border:1px black solid;" class="break">HighPromoter_PduD(1-20)_mCherry</td>
 
<td style="border:1px black solid;" class="break">HighPromoter_PduD(1-20)_mCherry</td>
 
<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213008"target="_blank"><b>BBa_K2213008</b></a></td>
 
<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213008"target="_blank"><b>BBa_K2213008</b></a></td>
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<td style="border:1px black solid;">Part Description.</td>
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K562001" target="_blank"><b>BBa_K562001</b></a>, with the illegal XbaI site removed, and with a high promoter (<a href="http://parts.igem.org/Part:BBa_J23104" target="_blank"><b>BBa_J23104</b></a>), and mCherry added.</td>
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<td style="border:1px black solid;">Basic</td>
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<td style="border:1px black solid;">Basic - Improved</td>
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</table>
 
</table>
  
 
</center>
 
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</div>
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<div class="plan" style="padding: 0px 8vw; background-color: #f5fcff!important">
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<a class="floatleft1 project projectlink" href= " https://2017.igem.org/Team:Manchester/Part_Collection " ><img src="https://static.igem.org/mediawiki/2017/3/3c/T--Manchester--NEXTARROWPARTS17.jpg"></a>
  
 +
<a class="floatright1 project projectlink" href= " https://2017.igem.org/Team:Manchester/Composite_Part " ><img src="https://static.igem.org/mediawiki/2017/1/11/T--Manchester--BACKARROWPARTSMAN17.jpg"></a>
  
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<br>
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</div>
  
 
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</div>

Latest revision as of 21:07, 1 November 2017

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JUDGING FORM

Improved Part



f


Please visit our Part Collection page for ALL PARTS.


The Manchester iGEM 2017 team have improved part BBa_K562001, originally submitted by team Dundee 2011.

We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible.

We have also further improved this part by adding different Anderson promoters (Low BBa_J23105, Medium BBa_J23108, High BBa_J23104) to control the expression level of the localisation tag.

This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.


Part Name BioBrick Description Part Type
LowPromoter_PduD(1-20)_mCherry BBa_K2213006 BBa_K562001,with the illegal XbaI site removed, and with a low promoter (BBa_J23105), and mCherry added. Basic - Improved
MediumPromoter_PduD(1-20)_mCherry BBa_K2213007 BBa_K562001, with the illegal XbaI site removed, and with a medium promoter (BBa_J23108), and mCherry added. Basic - Improved
HighPromoter_PduD(1-20)_mCherry BBa_K2213008 BBa_K562001, with the illegal XbaI site removed, and with a high promoter (BBa_J23104), and mCherry added. Basic - Improved