Difference between revisions of "Team:Manchester/Improve"
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<body> | <body> | ||
| − | < | + | <div class="clear"></div> |
| + | <div class="plan" style="background-color: #f5fcff!important"> | ||
<div class="col-md-12 sectionfirst" style="background-color: #f5fcff!important"> | <div class="col-md-12 sectionfirst" style="background-color: #f5fcff!important"> | ||
<h2 class="border" style="margin-top: 5vh; text-align: center">Improved Part</h2> | <h2 class="border" style="margin-top: 5vh; text-align: center">Improved Part</h2> | ||
</div> | </div> | ||
| − | <div class="plan" style="padding: 0px | + | <div class="plan" style="padding: 0px 18vw; background-color: #f5fcff!important"> |
<br><br> | <br><br> | ||
| − | + | <center> | |
| + | <p style="color: #f5fcff!important">f</p><br> | ||
<p style="text-align: center; font-size: 20px!important"><i> Please visit our <a href="https://2017.igem.org/Team:Manchester/Part_Collection"><b>Part Collection</b></a> page for ALL PARTS.</i></p> | <p style="text-align: center; font-size: 20px!important"><i> Please visit our <a href="https://2017.igem.org/Team:Manchester/Part_Collection"><b>Part Collection</b></a> page for ALL PARTS.</i></p> | ||
| − | + | <br> | |
| − | <p style="text-align: center; font-size: 18px!important"> The Manchester iGEM 2017 team have improved part <a href="http:// | + | <p style="text-align: center; font-size: 18px!important"> The Manchester iGEM 2017 team have improved part <a href="http://parts.igem.org/Part:BBa_K562001"><b>BBa_K562001</b></a>, originally submitted by team <a href="https://2011.igem.org/Team:Dundee"></b>Dundee 2011</b></a>.</p> |
<p style="text-align: center; font-size: 18px!important">We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible. </p> | <p style="text-align: center; font-size: 18px!important">We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible. </p> | ||
| − | <p style="text-align: center; font-size: 18px!important">We have also further improved this part by adding different Anderson promoters (<a href="http://parts.igem.org/Part:BBa_J23105">Low BBa_J23105</a>, <a href="http://parts.igem.org/Part:BBa_J23108">Medium BBa_J23108</a>, <a href="http://parts.igem.org/Part:BBa_J23104">High BBa_J23104</a>) to control the expression level of the localisation tag. This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation. </p> | + | <p style="text-align: center; font-size: 18px!important">We have also further improved this part by adding different Anderson promoters (<a href="http://parts.igem.org/Part:BBa_J23105">Low BBa_J23105</a>, <a href="http://parts.igem.org/Part:BBa_J23108">Medium BBa_J23108</a>, <a href="http://parts.igem.org/Part:BBa_J23104">High BBa_J23104</a>) to control the expression level of the localisation tag.</p> |
| − | <br | + | |
| + | <p style="text-align: center; font-size: 18px!important">This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.</p> | ||
| + | <br> | ||
</div> | </div> | ||
| − | + | ||
| − | + | ||
<table class="table table-bordered table-striped" style="width: 1000px; max-width: 95vw"> | <table class="table table-bordered table-striped" style="width: 1000px; max-width: 95vw"> | ||
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<tr> | <tr> | ||
| − | <th style="border:1px black solid;background: # | + | <th style="border:1px black solid;background: #d4f4fc;"><b>Part Name</b></th> |
| − | <th style="border:1px black solid;background: # | + | <th style="border:1px black solid;background: #d4f4fc;"><b>BioBrick</b></th> |
| − | <th style="border:1px black solid;background: # | + | <th style="border:1px black solid;background: #d4f4fc;"><b>Description</b></th> |
| − | <th style="border:1px black solid;background: # | + | <th style="border:1px black solid;background: #d4f4fc;"><b>Part Type</b></th> |
</tr> | </tr> | ||
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<td style="border:1px black solid;" class="break">LowPromoter_PduD(1-20)_mCherry</td> | <td style="border:1px black solid;" class="break">LowPromoter_PduD(1-20)_mCherry</td> | ||
<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213006" target="_blank"><b>BBa_K2213006</b></a></td> | <td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213006" target="_blank"><b>BBa_K2213006</b></a></td> | ||
| − | <td style="border:1px black solid;">BBa_K562001 with the illegal XbaI site removed, and with a low promoter (BBa_J23105), and mCherry added.</td> | + | <td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K562001" target="_blank"><b>BBa_K562001</b></a>,with the illegal XbaI site removed, and with a low promoter (<a href="http://parts.igem.org/Part:BBa_J23105" target="_blank"><b>BBa_J23105</b></a>), and mCherry added.</td> |
<td style="border:1px black solid;">Basic - Improved</td> | <td style="border:1px black solid;">Basic - Improved</td> | ||
</tr> | </tr> | ||
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<td style="border:1px black solid;" class="break">MediumPromoter_PduD(1-20)_mCherry</td> | <td style="border:1px black solid;" class="break">MediumPromoter_PduD(1-20)_mCherry</td> | ||
<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213007"target="_blank"><b>BBa_K2213007</b></a></td> | <td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213007"target="_blank"><b>BBa_K2213007</b></a></td> | ||
| − | <td style="border:1px black solid;">BBa_K562001 with the illegal XbaI site removed, and with a medium promoter (BBa_J23108), and mCherry added.</td> | + | <td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K562001" target="_blank"><b>BBa_K562001</b></a>, with the illegal XbaI site removed, and with a medium promoter (<a href="http://parts.igem.org/Part:BBa_J23108" target="_blank"><b>BBa_J23108</b></a>), and mCherry added.</td> |
<td style="border:1px black solid;">Basic - Improved</td> | <td style="border:1px black solid;">Basic - Improved</td> | ||
</tr> | </tr> | ||
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<td style="border:1px black solid;" class="break">HighPromoter_PduD(1-20)_mCherry</td> | <td style="border:1px black solid;" class="break">HighPromoter_PduD(1-20)_mCherry</td> | ||
<td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213008"target="_blank"><b>BBa_K2213008</b></a></td> | <td style="border:1px black solid;" class="break"><a href="http://parts.igem.org/Part:BBa_K2213008"target="_blank"><b>BBa_K2213008</b></a></td> | ||
| − | <td style="border:1px black solid;">BBa_K562001 with the illegal XbaI site removed, and with a high promoter (BBa_J23104), and mCherry added.</td> | + | <td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K562001" target="_blank"><b>BBa_K562001</b></a>, with the illegal XbaI site removed, and with a high promoter (<a href="http://parts.igem.org/Part:BBa_J23104" target="_blank"><b>BBa_J23104</b></a>), and mCherry added.</td> |
<td style="border:1px black solid;">Basic - Improved</td> | <td style="border:1px black solid;">Basic - Improved</td> | ||
| − | |||
</table> | </table> | ||
</center> | </center> | ||
| + | <br> | ||
| + | <br> | ||
| + | </div> | ||
| + | <div class="plan" style="padding: 0px 8vw; background-color: #f5fcff!important"> | ||
| + | |||
| + | <a class="floatleft1 project projectlink" href= " https://2017.igem.org/Team:Manchester/Part_Collection " ><img src="https://static.igem.org/mediawiki/2017/3/3c/T--Manchester--NEXTARROWPARTS17.jpg"></a> | ||
| + | |||
| + | <a class="floatright1 project projectlink" href= " https://2017.igem.org/Team:Manchester/Composite_Part " ><img src="https://static.igem.org/mediawiki/2017/1/11/T--Manchester--BACKARROWPARTSMAN17.jpg"></a> | ||
| + | |||
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
</div> | </div> | ||
| + | |||
</div> | </div> | ||
</div> | </div> | ||
Latest revision as of 21:07, 1 November 2017
Improved Part
f
Please visit our Part Collection page for ALL PARTS.
The Manchester iGEM 2017 team have improved part BBa_K562001, originally submitted by team Dundee 2011.
We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible.
We have also further improved this part by adding different Anderson promoters (Low BBa_J23105, Medium BBa_J23108, High BBa_J23104) to control the expression level of the localisation tag.
This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.
| Part Name | BioBrick | Description | Part Type |
|---|---|---|---|
| LowPromoter_PduD(1-20)_mCherry | BBa_K2213006 | BBa_K562001,with the illegal XbaI site removed, and with a low promoter (BBa_J23105), and mCherry added. | Basic - Improved |
| MediumPromoter_PduD(1-20)_mCherry | BBa_K2213007 | BBa_K562001, with the illegal XbaI site removed, and with a medium promoter (BBa_J23108), and mCherry added. | Basic - Improved |
| HighPromoter_PduD(1-20)_mCherry | BBa_K2213008 | BBa_K562001, with the illegal XbaI site removed, and with a high promoter (BBa_J23104), and mCherry added. | Basic - Improved |
