Difference between revisions of "Team:Lethbridge/Experiments"

 
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<div><img src="https://static.igem.org/mediawiki/2017/f/f4/Banner_LAexperiments.png"  class="center fit"></div>
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<div><img src="https://static.igem.org/mediawiki/2017/f/f4/Banner_LAexperiments.png"  class="bannerImg"></div>
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<br>
 
<br>
  
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<div class="segmentDiv"><h2 class="segmentHeader">Protocols</h2></div>
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<h2 class="segmentHeader">Protocols</h2>
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<table id="protocolTable">
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    <table id="protocolTable">
 
     <tr>
 
     <tr>
 
         <td><a href="https://static.igem.org/mediawiki/2017/5/5f/Agarose_Gel_Extraction.pdf" target="_blank">Agarose Gel Extraction</a></td>
 
         <td><a href="https://static.igem.org/mediawiki/2017/5/5f/Agarose_Gel_Extraction.pdf" target="_blank">Agarose Gel Extraction</a></td>
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     <tr>
 
     <tr>
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         <td><a href="https://static.igem.org/mediawiki/2017/6/6f/DNA_Purification_from_Enzymatic_Reactions.pdf" target="_blank">DNA PUrification from Enzymatic Reactions</a></td>
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         <td><a href="https://static.igem.org/mediawiki/2017/6/6f/DNA_Purification_from_Enzymatic_Reactions.pdf" target="_blank">DNA Purification from Enzymatic Reactions</a></td>
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         <td><a href="https://static.igem.org/mediawiki/2017/5/51/In_vitro_transcription.pdf" target="_blank">In Vitro Transcription</a></td>
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         <td><a href="https://static.igem.org/mediawiki/2017/5/51/In_vitro_transcription.pdf" target="_blank"><i>In vitro</i> Transcription</a></td>
 
     </tr>
 
     </tr>
 
      
 
      
 
     <tr>
 
     <tr>
 
         <td><a href="https://static.igem.org/mediawiki/2017/b/ba/LB_Media.pdf" target="_blank">LB Media</a></td>
 
         <td><a href="https://static.igem.org/mediawiki/2017/b/ba/LB_Media.pdf" target="_blank">LB Media</a></td>
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         <td><a href="https://static.igem.org/mediawiki/2017/6/6c/Ligation_protocol.pdf" target="_blank">Ligations</a></td>
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         <td><a href="https://static.igem.org/mediawiki/2017/9/9d/Ligation.pdf" target="_blank">Ligations</a></td>
 
     </tr>
 
     </tr>
 
      
 
      
 
     <tr>
 
     <tr>
 
         <td><a href="https://static.igem.org/mediawiki/2017/6/68/PCR_Amplification.pdf" target="_blank">PCR Amplification</a></td>
 
         <td><a href="https://static.igem.org/mediawiki/2017/6/68/PCR_Amplification.pdf" target="_blank">PCR Amplification</a></td>
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         <td><a href="https://static.igem.org/mediawiki/2017/e/e5/PCR_Product_Purification.pdf" target="_blank">PCR product Purification</a></td>
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         <td><a href="https://static.igem.org/mediawiki/2017/e/e5/PCR_Product_Purification.pdf" target="_blank">PCR Product Purification</a></td>
 
     </tr>
 
     </tr>
 
      
 
      
 
     <tr>
 
     <tr>
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         <td><a href="https://static.igem.org/mediawiki/2017/d/df/PhenolChloroform_extraction_%26_ethanol_percipitation.pdf" target="_blank">Phenol/Chloroform Extraction and Ethanol Precipitation</a></td>
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         <td><a href="https://static.igem.org/mediawiki/2017/f/f2/PhenolFChloroform_extraction_%26_ethanol_percipitation.pdf" target="_blank">Phenol/Chloroform Extraction and Ethanol Precipitation</a></td>
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         <td><a href="https://static.igem.org/mediawiki/2017/e/eb/PJET_Cloning.pdf">pJET Cloning</a></td>
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         <td><a href="https://static.igem.org/mediawiki/2017/e/eb/PJET_Cloning.pdf" target="_blank">pJET Cloning</a></td>
 
     </tr>
 
     </tr>
 
      
 
      
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     <tr class="backGrey">
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         <td><a href="https://static.igem.org/mediawiki/2017/0/08/Plasmid_Miniprep.pdf">Plasmid Miniprep</a></td>
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         <td><a href="https://static.igem.org/mediawiki/2017/0/08/Plasmid_Miniprep.pdf" target="_blank">Plasmid Miniprep</a></td>
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         <td><a href="https://static.igem.org/mediawiki/2017/7/78/Preparation_of_Chemical_Competent_Cells.pdf">Preparation of Chemical Competent Cells</a></td>
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         <td><a href="https://static.igem.org/mediawiki/2017/7/78/Preparation_of_Chemical_Competent_Cells.pdf" target="_blank">Preparation of Chemical Competent Cells</a></td>
 
     </tr>
 
     </tr>
 
      
 
      
 
     <tr>
 
     <tr>
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         <td><a href="https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf">Protein Overexpression</a></td>
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         <td><a href="https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf" target="_blank">Protein Overexpression</a></td>
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         <td><a href="https://static.igem.org/mediawiki/2017/5/5f/PURExpress.pdf">PURExpress NEB Kit</a></td>
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         <td><a href="https://static.igem.org/mediawiki/2017/5/5f/PURExpress.pdf" target="_blank">PURExpress NEB Kit</a></td>
 
     </tr>
 
     </tr>
 
      
 
      
 
     <tr>
 
     <tr>
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         <td><a href="https://static.igem.org/mediawiki/2017/f/fc/Purification_of_Poly-Histidine_Tagged_Proteins.pdf">Purification of Poly-Histidine Tagged Proteins</a></td>
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         <td><a href="https://static.igem.org/mediawiki/2017/f/fc/Purification_of_Poly-Histidine_Tagged_Proteins.pdf" target="_blank">Purification of HexaHistidine Tagged Proteins</a></td>
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         <td><a href="https://static.igem.org/mediawiki/2017/1/10/Restriction_Enzyme_Digest_and_Dephosphorylation.pdf">Restriction Enzyme Digest and Dephosphorylation</a></td>
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         <td><a href="https://static.igem.org/mediawiki/2017/1/10/Restriction_Enzyme_Digest_and_Dephosphorylation.pdf" target="_blank">Restriction Enzyme Digest and Dephosphorylation</a></td>
 
     </tr>
 
     </tr>
 
      
 
      
 
     <tr>
 
     <tr>
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         <td><a href="https://static.igem.org/mediawiki/2017/2/2a/T7-RNA_Polymerase_Purification.pdf">T7- RNA Polymerase Purification</a></td>
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         <td><a href="https://static.igem.org/mediawiki/2017/2/2a/T7-RNA_Polymerase_Purification.pdf" target="_blank">T7- RNA Polymerase Purification</a></td>
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         <td><a href="https://static.igem.org/mediawiki/2017/a/a6/Transformation.pdf">Transformation</a></td>
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         <td><a href="https://static.igem.org/mediawiki/2017/a/a6/Transformation.pdf" target="_blank">Transformation</a></td>
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    </tr>
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    <tr>
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        <td><a href="https://static.igem.org/mediawiki/2017/c/cd/MS2-tRNA_Complex_Purification.pdf" target="_blank">MS2-tRNA Affinity Chromatography Purification</a></td>
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        <td></td>
 
     </tr>
 
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     <td>IPTG</td>
 
     <td>IPTG</td>
 
   <td>Prefix Forward: 5’GAATTCGCGGCCGCTTCTAG3’</td>
 
   <td>Prefix Forward: 5’GAATTCGCGGCCGCTTCTAG3’</td>
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</td>
 
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td>Phusion Polymerase Master Mix</td>
 
     <td>Phusion Polymerase Master Mix</td>
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     <td>Kanomycin/Chloramphenicol/Ampicillin </td>
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     <td>Kanomycin / Chloramphenicol / Ampicillin </td>
 
   <td>Prefix Reverse: 5’CTGCAGCGGCCGCTACTAGTA3’</td>
 
   <td>Prefix Reverse: 5’CTGCAGCGGCCGCTACTAGTA3’</td>
 
   </tr>
 
   </tr>
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     <td>T7 DNA Polymerase</td>
 
     <td>T7 DNA Polymerase</td>
 
     <td>T7 RNA Polymerase Purification Buffers</td>
 
     <td>T7 RNA Polymerase Purification Buffers</td>
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     <td>Dialyzation Membrane</td>
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     <td>Dialysis Tubing</td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
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</tr>
 
<tr>
 
<tr>
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       <td>RNAse A</td>
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       <td>RNase A</td>
 
       <td>SDS-PAGE Reagents</td>
 
       <td>SDS-PAGE Reagents</td>
 
     <td></td>
 
     <td></td>
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</tr>
 
</tr>
 
<tr>
 
<tr>
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     <td>RNAse Inhibitor</td>
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     <td>RNase Inhibitor</td>
 
     <td>Agar</td>
 
     <td>Agar</td>
 
     <td></td>
 
     <td></td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
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     <td>DNAseI</td>
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     <td>DNaseI</td>
 
     <td>Tryptone</td>
 
     <td>Tryptone</td>
 
     <td></td>
 
     <td></td>
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     <td>TAKMz</td>
 
     <td>TAKMz</td>
 
     <td> </td>
 
     <td> </td>
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<tr>
 
<tr>
 
     <td></td>
 
     <td></td>
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Latest revision as of 03:07, 2 November 2017

top

Protocols


Products/Reagents

Enzymes Buffers/Reagents Other Products
Shrimp Alkaline Phosphatase Affinity Chromatography Buffers Ni²+ Affinity Resin
DNA Blunting Enzyme IPTG Prefix Forward: 5’GAATTCGCGGCCGCTTCTAG3’
Phusion Polymerase Master Mix Kanomycin / Chloramphenicol / Ampicillin Prefix Reverse: 5’CTGCAGCGGCCGCTACTAGTA3’
PFU Polymerase Dimethyl Sulfur Dioxide Prefix Forward +6:5’GGCTCAGAATTCGCGGCCGCTTCTAGAG3’
T7 RNA Polymerase Competent Cell Buffers Suffix Reverse +6: 5’CTGGACCTGCAGCGGCCGCTACTAGTA3’
T7 DNA Polymerase T7 RNA Polymerase Purification Buffers Dialysis Tubing
PURExpressKit (New England Biolabs) Phenol
XbaI (NEB) Chloroform
SpeI (NEB) 10x PFU Buffer
PstI (NEB) NTP
EcoRI (NEB) GMP
Dream Taq DNA Polymerase 10x Taq Buffer
Taq DNA Polymerase Ligase Buffer
RNase A SDS-PAGE Reagents
TraB Urea PAGE reagents
iPPase Agarose
RNase Inhibitor Agar
DNaseI Tryptone
Yeast Extract
TAKMz
BioBasic Plasmid DNA miniprep Kit
Imidazole