Difference between revisions of "Team:Lethbridge/Experiments"
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/* PRODUCT REAGENT TABLE ENDS */ | /* PRODUCT REAGENT TABLE ENDS */ | ||
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<body> | <body> | ||
<div><img src="https://static.igem.org/mediawiki/2017/f/f4/Banner_LAexperiments.png" class="bannerImg"></div> | <div><img src="https://static.igem.org/mediawiki/2017/f/f4/Banner_LAexperiments.png" class="bannerImg"></div> | ||
| − | <div id="allContent"> | + | <div id="allContent"><!--div to set margin for all content but header and footer image --> |
<br> | <br> | ||
| − | <div class="segmentDiv"> | + | <div class="segmentDiv"><h2 class="segmentHeader">Protocols</h2></div> |
| − | <h2 class="segmentHeader">Protocols</h2> | + | |
| − | </div> | + | |
<center> | <center> | ||
| − | <div> | + | <div> |
| − | <table id="protocolTable"> | + | |
| + | <table id="protocolTable"> | ||
<tr> | <tr> | ||
<td><a href="https://static.igem.org/mediawiki/2017/5/5f/Agarose_Gel_Extraction.pdf" target="_blank">Agarose Gel Extraction</a></td> | <td><a href="https://static.igem.org/mediawiki/2017/5/5f/Agarose_Gel_Extraction.pdf" target="_blank">Agarose Gel Extraction</a></td> | ||
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<tr> | <tr> | ||
| − | <td><a href="https://static.igem.org/mediawiki/2017/6/6f/DNA_Purification_from_Enzymatic_Reactions.pdf" target="_blank">DNA | + | <td><a href="https://static.igem.org/mediawiki/2017/6/6f/DNA_Purification_from_Enzymatic_Reactions.pdf" target="_blank">DNA Purification from Enzymatic Reactions</a></td> |
| − | <td><a href="https://static.igem.org/mediawiki/2017/5/51/In_vitro_transcription.pdf" target="_blank">In | + | <td><a href="https://static.igem.org/mediawiki/2017/5/51/In_vitro_transcription.pdf" target="_blank"><i>In vitro</i> Transcription</a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="https://static.igem.org/mediawiki/2017/b/ba/LB_Media.pdf" target="_blank">LB Media</a></td> | <td><a href="https://static.igem.org/mediawiki/2017/b/ba/LB_Media.pdf" target="_blank">LB Media</a></td> | ||
| − | <td><a href="https://static.igem.org/mediawiki/2017/ | + | <td><a href="https://static.igem.org/mediawiki/2017/9/9d/Ligation.pdf" target="_blank">Ligations</a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="https://static.igem.org/mediawiki/2017/6/68/PCR_Amplification.pdf" target="_blank">PCR Amplification</a></td> | <td><a href="https://static.igem.org/mediawiki/2017/6/68/PCR_Amplification.pdf" target="_blank">PCR Amplification</a></td> | ||
| − | <td><a href="https://static.igem.org/mediawiki/2017/e/e5/PCR_Product_Purification.pdf" target="_blank">PCR | + | <td><a href="https://static.igem.org/mediawiki/2017/e/e5/PCR_Product_Purification.pdf" target="_blank">PCR Product Purification</a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href="https://static.igem.org/mediawiki/2017/ | + | <td><a href="https://static.igem.org/mediawiki/2017/f/f2/PhenolFChloroform_extraction_%26_ethanol_percipitation.pdf" target="_blank">Phenol/Chloroform Extraction and Ethanol Precipitation</a></td> |
<td><a href="https://static.igem.org/mediawiki/2017/e/eb/PJET_Cloning.pdf" target="_blank">pJET Cloning</a></td> | <td><a href="https://static.igem.org/mediawiki/2017/e/eb/PJET_Cloning.pdf" target="_blank">pJET Cloning</a></td> | ||
</tr> | </tr> | ||
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<td><a href="https://static.igem.org/mediawiki/2017/2/2a/T7-RNA_Polymerase_Purification.pdf" target="_blank">T7- RNA Polymerase Purification</a></td> | <td><a href="https://static.igem.org/mediawiki/2017/2/2a/T7-RNA_Polymerase_Purification.pdf" target="_blank">T7- RNA Polymerase Purification</a></td> | ||
<td><a href="https://static.igem.org/mediawiki/2017/a/a6/Transformation.pdf" target="_blank">Transformation</a></td> | <td><a href="https://static.igem.org/mediawiki/2017/a/a6/Transformation.pdf" target="_blank">Transformation</a></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><a href="https://static.igem.org/mediawiki/2017/c/cd/MS2-tRNA_Complex_Purification.pdf" target="_blank">MS2-tRNA Affinity Chromatography Purification</a></td> | ||
| + | <td></td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
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<td>T7 DNA Polymerase</td> | <td>T7 DNA Polymerase</td> | ||
<td>T7 RNA Polymerase Purification Buffers</td> | <td>T7 RNA Polymerase Purification Buffers</td> | ||
| − | <td> | + | <td>Dialysis Tubing</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>RNase A</td> |
<td>SDS-PAGE Reagents</td> | <td>SDS-PAGE Reagents</td> | ||
<td></td> | <td></td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>RNase Inhibitor</td> |
<td>Agar</td> | <td>Agar</td> | ||
<td></td> | <td></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>DNaseI</td> |
<td>Tryptone</td> | <td>Tryptone</td> | ||
<td></td> | <td></td> | ||
Latest revision as of 03:07, 2 November 2017
Protocols
Products/Reagents
| Enzymes | Buffers/Reagents | Other Products |
|---|---|---|
| Shrimp Alkaline Phosphatase | Affinity Chromatography Buffers | Ni²+ Affinity Resin |
| DNA Blunting Enzyme | IPTG | Prefix Forward: 5’GAATTCGCGGCCGCTTCTAG3’ |
| Phusion Polymerase Master Mix | Kanomycin / Chloramphenicol / Ampicillin | Prefix Reverse: 5’CTGCAGCGGCCGCTACTAGTA3’ |
| PFU Polymerase | Dimethyl Sulfur Dioxide | Prefix Forward +6:5’GGCTCAGAATTCGCGGCCGCTTCTAGAG3’ |
| T7 RNA Polymerase | Competent Cell Buffers | Suffix Reverse +6: 5’CTGGACCTGCAGCGGCCGCTACTAGTA3’ |
| T7 DNA Polymerase | T7 RNA Polymerase Purification Buffers | Dialysis Tubing |
| PURExpressKit (New England Biolabs) | Phenol | |
| XbaI (NEB) | Chloroform | |
| SpeI (NEB) | 10x PFU Buffer | |
| PstI (NEB) | NTP | |
| EcoRI (NEB) | GMP | |
| Dream Taq DNA Polymerase | 10x Taq Buffer | |
| Taq DNA Polymerase | Ligase Buffer | |
| RNase A | SDS-PAGE Reagents | |
| TraB | Urea PAGE reagents | |
| iPPase | Agarose | |
| RNase Inhibitor | Agar | |
| DNaseI | Tryptone | |
| Yeast Extract | ||
| TAKMz | ||
| BioBasic Plasmid DNA miniprep Kit | ||
| Imidazole |
