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{{Team:Lethbridge/navbar}} | {{Team:Lethbridge/navbar}} | ||
{{Team:Lethbridge/assets/allpages.css}} | {{Team:Lethbridge/assets/allpages.css}} | ||
+ | {{Team:Lethbridge/top}} | ||
<html> | <html> | ||
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<tr> | <tr> | ||
− | <td><a href="https://static.igem.org/mediawiki/2017/6/6f/DNA_Purification_from_Enzymatic_Reactions.pdf" target="_blank">DNA | + | <td><a href="https://static.igem.org/mediawiki/2017/6/6f/DNA_Purification_from_Enzymatic_Reactions.pdf" target="_blank">DNA Purification from Enzymatic Reactions</a></td> |
− | <td><a href="https://static.igem.org/mediawiki/2017/5/51/In_vitro_transcription.pdf" target="_blank">In | + | <td><a href="https://static.igem.org/mediawiki/2017/5/51/In_vitro_transcription.pdf" target="_blank"><i>In vitro</i> Transcription</a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="https://static.igem.org/mediawiki/2017/b/ba/LB_Media.pdf" target="_blank">LB Media</a></td> | <td><a href="https://static.igem.org/mediawiki/2017/b/ba/LB_Media.pdf" target="_blank">LB Media</a></td> | ||
− | <td><a href="https://static.igem.org/mediawiki/2017/ | + | <td><a href="https://static.igem.org/mediawiki/2017/9/9d/Ligation.pdf" target="_blank">Ligations</a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="https://static.igem.org/mediawiki/2017/6/68/PCR_Amplification.pdf" target="_blank">PCR Amplification</a></td> | <td><a href="https://static.igem.org/mediawiki/2017/6/68/PCR_Amplification.pdf" target="_blank">PCR Amplification</a></td> | ||
− | <td><a href="https://static.igem.org/mediawiki/2017/e/e5/PCR_Product_Purification.pdf" target="_blank">PCR | + | <td><a href="https://static.igem.org/mediawiki/2017/e/e5/PCR_Product_Purification.pdf" target="_blank">PCR Product Purification</a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td><a href="https://static.igem.org/mediawiki/2017/ | + | <td><a href="https://static.igem.org/mediawiki/2017/f/f2/PhenolFChloroform_extraction_%26_ethanol_percipitation.pdf" target="_blank">Phenol/Chloroform Extraction and Ethanol Precipitation</a></td> |
<td><a href="https://static.igem.org/mediawiki/2017/e/eb/PJET_Cloning.pdf" target="_blank">pJET Cloning</a></td> | <td><a href="https://static.igem.org/mediawiki/2017/e/eb/PJET_Cloning.pdf" target="_blank">pJET Cloning</a></td> | ||
</tr> | </tr> | ||
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<td><a href="https://static.igem.org/mediawiki/2017/a/a6/Transformation.pdf" target="_blank">Transformation</a></td> | <td><a href="https://static.igem.org/mediawiki/2017/a/a6/Transformation.pdf" target="_blank">Transformation</a></td> | ||
</tr> | </tr> | ||
− | <tr> | + | <tr> |
<td><a href="https://static.igem.org/mediawiki/2017/c/cd/MS2-tRNA_Complex_Purification.pdf" target="_blank">MS2-tRNA Affinity Chromatography Purification</a></td> | <td><a href="https://static.igem.org/mediawiki/2017/c/cd/MS2-tRNA_Complex_Purification.pdf" target="_blank">MS2-tRNA Affinity Chromatography Purification</a></td> | ||
<td></td> | <td></td> | ||
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<td>T7 DNA Polymerase</td> | <td>T7 DNA Polymerase</td> | ||
<td>T7 RNA Polymerase Purification Buffers</td> | <td>T7 RNA Polymerase Purification Buffers</td> | ||
− | <td> | + | <td>Dialysis Tubing</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td> | + | <td>RNase A</td> |
<td>SDS-PAGE Reagents</td> | <td>SDS-PAGE Reagents</td> | ||
<td></td> | <td></td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td> | + | <td>RNase Inhibitor</td> |
<td>Agar</td> | <td>Agar</td> | ||
<td></td> | <td></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td> | + | <td>DNaseI</td> |
<td>Tryptone</td> | <td>Tryptone</td> | ||
<td></td> | <td></td> |
Latest revision as of 03:07, 2 November 2017
Protocols
Products/Reagents
Enzymes | Buffers/Reagents | Other Products |
---|---|---|
Shrimp Alkaline Phosphatase | Affinity Chromatography Buffers | Ni²+ Affinity Resin |
DNA Blunting Enzyme | IPTG | Prefix Forward: 5’GAATTCGCGGCCGCTTCTAG3’ |
Phusion Polymerase Master Mix | Kanomycin / Chloramphenicol / Ampicillin | Prefix Reverse: 5’CTGCAGCGGCCGCTACTAGTA3’ |
PFU Polymerase | Dimethyl Sulfur Dioxide | Prefix Forward +6:5’GGCTCAGAATTCGCGGCCGCTTCTAGAG3’ |
T7 RNA Polymerase | Competent Cell Buffers | Suffix Reverse +6: 5’CTGGACCTGCAGCGGCCGCTACTAGTA3’ |
T7 DNA Polymerase | T7 RNA Polymerase Purification Buffers | Dialysis Tubing |
PURExpressKit (New England Biolabs) | Phenol | |
XbaI (NEB) | Chloroform | |
SpeI (NEB) | 10x PFU Buffer | |
PstI (NEB) | NTP | |
EcoRI (NEB) | GMP | |
Dream Taq DNA Polymerase | 10x Taq Buffer | |
Taq DNA Polymerase | Ligase Buffer | |
RNase A | SDS-PAGE Reagents | |
TraB | Urea PAGE reagents | |
iPPase | Agarose | |
RNase Inhibitor | Agar | |
DNaseI | Tryptone | |
Yeast Extract | ||
TAKMz | ||
BioBasic Plasmid DNA miniprep Kit | ||
Imidazole |