Difference between revisions of "Team:KU Leuven"

Line 27: Line 27:
 
.igem_2017_content_wrapper {
 
.igem_2017_content_wrapper {
 
padding-bottom: 0;
 
padding-bottom: 0;
 +
}
 +
#sine {
 +
position: relative;
 +
}
 +
#sine > svg {
 +
position: absolute;
 +
 
}
 
}
 
</style>
 
</style>
Line 37: Line 44:
 
</div>
 
</div>
 
<div class="overlay">
 
<div class="overlay">
<svg xmlns="http://www.w3.org/2000/svg" version="1.1" width='100%' height='100px' viewBox="0 0 200 80">
+
<div id="sine">
<path
+
<svg xmlns="http://www.w3.org/2000/svg" version="1.1" width='100%' height='100px' viewBox="0 0 200 80">
style="fill:none;stroke:#cc3333;stroke-width:2;stroke-linecap:round;stroke-linejoin:miter;stroke-miterlimit:4;stroke-opacity:1;stroke-dasharray:400;stroke-dashoffset:0"
+
<path
data-500="stroke-dashoffset:400;" data-800="stroke-dashoffset:0;"
+
style="fill:none;stroke:#aaaaaa;stroke-width:2;" d="m 12,35 c 5,20 10,20 10,20 0,0 5,0 10,-20 5,-20 10,-20 10,-20 0,0 5,0 10,20 5,20 10,20 10,20 0,0 5,0 10,-20 5,-20 10,-20 10,-20 0,0 5,0 10,20 5,20 10,20 10,20 0,0 5,0 10,-20 5,-20 10,-20 10,-20 0,0 5,0 10,20 5,20 10,20 10,20 0,0 5,0 10,-20 5,-20 10,-20 10,-20 0,0 5,0 10,20">
d="m 12,35 c 5,20 10,20 10,20 0,0 5,0 10,-20 5,-20 10,-20 10,-20 0,0 5,0 10,20 5,20 10,20 10,20 0,0 5,0 10,-20 5,-20 10,-20 10,-20 0,0 5,0 10,20 5,20 10,20 10,20 0,0 5,0 10,-20 5,-20 10,-20 10,-20 0,0 5,0 10,20 5,20 10,20 10,20 0,0 5,0 10,-20 5,-20 10,-20 10,-20 0,0 5,0 10,20"
+
</path>
/>
+
 
</svg>
 
</svg>
<div class="container">
+
<svg xmlns="http://www.w3.org/2000/svg" version="1.1" width='100%' height='100px' viewBox="0 0 200 80">
<div class="description">
+
<path
<h2>Description</h2>
+
style="fill:none;stroke:#cc3333;stroke-width:2;stroke-linecap:round;stroke-linejoin:miter;stroke-miterlimit:4;stroke-opacity:1;stroke-dasharray:400;stroke-dashoffset:0"
<p>HEKcite! Inspired by the human heart rhythm, we aim to create an electrophysiological oscillator from eukaryotic cells. Rhythmic contraction of heart cells is coordinated by a small group of cells called the sinus node that have an intrinsic frequency of de- and repolarization. This frequency of electrical oscillation is influenced by environmental parameters as well as certain molecular substrates. The oscillator that is proposed here consists of genetically modified excitable Human Embryonic Kidney (HEK) cells, altered to contain the intrinsic pacemaker ability found in sinus cells. As witnessed in heart cells, the rhythm would be dependent on substrate-activated ion channels in the membrane. As there is a great variety of ion channels available in nature, the oscillator could be modified to measure concentrations of many specific substrates. By integrating a certain ion channel into the oscillating system, specificity for a substrate can be chosen.Building an electrical oscillator from cells has several advantages. Extra- or intracellular changes that influence the conductance of ion channels in the membrane have an immediate impact on the frequency of oscillation. Once these cells are connected to each other (by for example gap-junctions), they generate an electrical signal that can easily be measured from a distance and non-invasively—similar to the way electrocardiography (ECG) and electroencephalography (EEG) measure electrical activity in the heart and brain respectively. A multi-purpose sensor suitable for this system could be developed for medical and biotechnological applications. One such application is the measurement of drugs that interact with ion channels, such as anti-epileptics or a certain class of immunosuppressants.
+
data-500="stroke-dashoffset:400;" data-800="stroke-dashoffset:0;"
</p>
+
d="m 12,35 c 5,20 10,20 10,20 0,0 5,0 10,-20 5,-20 10,-20 10,-20 0,0 5,0 10,20 5,20 10,20 10,20 0,0 5,0 10,-20 5,-20 10,-20 10,-20 0,0 5,0 10,20 5,20 10,20 10,20 0,0 5,0 10,-20 5,-20 10,-20 10,-20 0,0 5,0 10,20 5,20 10,20 10,20 0,0 5,0 10,-20 5,-20 10,-20 10,-20 0,0 5,0 10,20">
</div>
+
</path>
<div class="education">
+
</svg>
<h2>Education</h2>
+
</div>
<p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod
+
<div class="container">
tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam,
+
<div class="description">
quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo
+
<h2>Description</h2>
consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse
+
<p>HEKcite! Inspired by the human heart rhythm, we aim to create an electrophysiological oscillator from eukaryotic cells. Rhythmic contraction of heart cells is coordinated by a small group of cells called the sinus node that have an intrinsic frequency of de- and repolarization. This frequency of electrical oscillation is influenced by environmental parameters as well as certain molecular substrates. The oscillator that is proposed here consists of genetically modified excitable Human Embryonic Kidney (HEK) cells, altered to contain the intrinsic pacemaker ability found in sinus cells. As witnessed in heart cells, the rhythm would be dependent on substrate-activated ion channels in the membrane. As there is a great variety of ion channels available in nature, the oscillator could be modified to measure concentrations of many specific substrates. By integrating a certain ion channel into the oscillating system, specificity for a substrate can be chosen.Building an electrical oscillator from cells has several advantages. Extra- or intracellular changes that influence the conductance of ion channels in the membrane have an immediate impact on the frequency of oscillation. Once these cells are connected to each other (by for example gap-junctions), they generate an electrical signal that can easily be measured from a distance and non-invasively—similar to the way electrocardiography (ECG) and electroencephalography (EEG) measure electrical activity in the heart and brain respectively. A multi-purpose sensor suitable for this system could be developed for medical and biotechnological applications. One such application is the measurement of drugs that interact with ion channels, such as anti-epileptics or a certain class of immunosuppressants.
cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non
+
</p>
proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
+
</div>
</p>
+
<div class="education">
</div>
+
<h2>Education</h2>
<div class="humanpractices">
+
<p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod
<h2>Human Practices</h2>
+
tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam,
<p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod
+
quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo
tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam,
+
consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse
quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo
+
cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non
consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse
+
proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non
+
</p>
proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
+
</div>
</p>
+
<div class="humanpractices">
</div>
+
<h2>Human Practices</h2>
<div class="game">
+
<p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod
<h2>Game</h2>
+
tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam,
<p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod
+
quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo
tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam,
+
consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse
quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo
+
cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non
consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse
+
proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non
+
</p>
proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
+
</div>
</p>
+
<div class="game">
</div>
+
<h2>Game</h2>
 +
<p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod
 +
tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam,
 +
quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo
 +
consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse
 +
cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non
 +
proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
 +
</p>
 
</div>
 
</div>
 
</div>
 
</div>
 
</div>
 
</div>
<script type="text/javascript">
+
</div>
var s = skrollr.init();
+
<script type="text/javascript">
</script>
+
var s = skrollr.init();
 +
</script>
 
 
 
</html>
 
</html>
  
 
{{KU_Leuven_footer}}
 
{{KU_Leuven_footer}}

Revision as of 09:44, 25 July 2017

Description

HEKcite! Inspired by the human heart rhythm, we aim to create an electrophysiological oscillator from eukaryotic cells. Rhythmic contraction of heart cells is coordinated by a small group of cells called the sinus node that have an intrinsic frequency of de- and repolarization. This frequency of electrical oscillation is influenced by environmental parameters as well as certain molecular substrates. The oscillator that is proposed here consists of genetically modified excitable Human Embryonic Kidney (HEK) cells, altered to contain the intrinsic pacemaker ability found in sinus cells. As witnessed in heart cells, the rhythm would be dependent on substrate-activated ion channels in the membrane. As there is a great variety of ion channels available in nature, the oscillator could be modified to measure concentrations of many specific substrates. By integrating a certain ion channel into the oscillating system, specificity for a substrate can be chosen.Building an electrical oscillator from cells has several advantages. Extra- or intracellular changes that influence the conductance of ion channels in the membrane have an immediate impact on the frequency of oscillation. Once these cells are connected to each other (by for example gap-junctions), they generate an electrical signal that can easily be measured from a distance and non-invasively—similar to the way electrocardiography (ECG) and electroencephalography (EEG) measure electrical activity in the heart and brain respectively. A multi-purpose sensor suitable for this system could be developed for medical and biotechnological applications. One such application is the measurement of drugs that interact with ion channels, such as anti-epileptics or a certain class of immunosuppressants.

Education

Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.

Human Practices

Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.

Game

Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.