Line 96: | Line 96: | ||
= Microfluidics = | = Microfluidics = | ||
− | In our experiment, we want to | + | In our experiment, we want to study the neural network activity and behavioral response of Caenorhabditis elegans under light stimuli of specific wavelengths. Thus, we need to design an accurate and user-friendly platform for studying the collective behavior of worms with high throughput as well as live neuron-level observations under natural conditions. |
+ | |||
− | + | To meet these design goals, we designed three microfluidic chips: the Selection Chip to select worms of appropriate sizes, the Gaussian Plate to monitor changes in the worm’s group behavior, and the Immobilization Plate to observe live neuron activities without anesthetization. (Fig.1) | |
− | {{SUSTech_Image_Center_fill-width | filename=T--SUSTech_Shenzhen--Microfluidics--fig1overview.png |width=1000px| caption=<B>Fig. 1 | + | |
+ | {{SUSTech_Image_Center_fill-width | filename=T--SUSTech_Shenzhen--Microfluidics--fig1overview.png |width=1000px| caption=<B>Fig. 1 Three microfluidic systems in our experiment. A) </B>Design of the Selection Chip and the Gaussian Chip.<B> B) </B>The Selection Chip and the Gaussian Chip, fabricated on the same substrate <B>C)</B> Design of the Immobilization Chip.<B> D)</B> The Immobilization Chip.}} | ||
<html><a target="_black" href="https://2017.igem.org/Team:SUSTech_Shenzhen/Hardware/Microfluidics" class="btn btn-default"><i class="ion-arrow-right-c"></i> Detailed Microfluidics</a></html> | <html><a target="_black" href="https://2017.igem.org/Team:SUSTech_Shenzhen/Hardware/Microfluidics" class="btn btn-default"><i class="ion-arrow-right-c"></i> Detailed Microfluidics</a></html> |
Revision as of 18:23, 1 November 2017
Contents
Microfluidics
In our experiment, we want to study the neural network activity and behavioral response of Caenorhabditis elegans under light stimuli of specific wavelengths. Thus, we need to design an accurate and user-friendly platform for studying the collective behavior of worms with high throughput as well as live neuron-level observations under natural conditions.
To meet these design goals, we designed three microfluidic chips: the Selection Chip to select worms of appropriate sizes, the Gaussian Plate to monitor changes in the worm’s group behavior, and the Immobilization Plate to observe live neuron activities without anesthetization. (Fig.1)
Light Modulator
Multiple devices of optics are designed and created for the various experiment requirements, such as, stimulate neuron of C. elegans, train C. elegans and induce C. elegans move in a special direction. All devices attempts to modulate the spatio-temporal pattern in an elegant and effective way. We constructed projector light source and modulate mercury lamp. Just let us start to play with light.
Projector Light Source
We use 395nm light to activate calcium indicator protein GEM-GECO by Lumencor LED Illuminator. Then CoChR and Chrimson are activated by blue light and red light from LCD projector. LCD projector and LED Illuminator are merged by a double LH Adapter contained a semi-transparent mirror, which connect to microscope. So we can use these two light source in the same time.
We try develop a more powerful and hackable open source software suite called ColorMapping to track and activate multi C. eleganss or cell independently in one eye-filed. User can modify multi color, intensity, time, locations of light alternately in GUI. ColorMapping can be found in GitHub, which still is developing.
Arduino Modulate Mercury Lamp
A simple and effective device to output pulse of certain wavelength of light. On time and off time of pulse is custom by Arduino. Wavelength of light is changed by replacing filter before beam expander.