Difference between revisions of "Team:Manchester/Improve"
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| − | <p>We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible. < | + | <p>We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible. </p> |
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| − | <p>We have also further improved this part by adding different Anderson promoters (<a href="http://parts.igem.org/Part:BBa_J23105">Low BBa_J23105<a/>, <a href="http://parts.igem.org/Part:BBa_J23108">Medium BBa_J23108<a/>, <a href="http://parts.igem.org/Part:BBa_J23104">High BBa_J23104<a/>) to control the expression level of the localisation tag. This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation. <p | + | <p>We have also further improved this part by adding different Anderson promoters (<a href="http://parts.igem.org/Part:BBa_J23105">Low BBa_J23105<a/>, <a href="http://parts.igem.org/Part:BBa_J23108">Medium BBa_J23108<a/>, <a href="http://parts.igem.org/Part:BBa_J23104">High BBa_J23104<a/>) to control the expression level of the localisation tag. This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation. </p> |
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<table style="width: 1000px; max-width: 95vw"> | <table style="width: 1000px; max-width: 95vw"> | ||
Revision as of 19:53, 22 October 2017
Improved Parts
Please visit our Part Collection page for ALL PARTS.
The Manchester iGEM 2017 team have improved part BBa_K562001, originally submitted by team Dundee 2011.
We have removed the illegal XbaI site at 179 in BBa_K562001 to make it biobrick compatible.
We have also further improved this part by adding different Anderson promoters (Low BBa_J23105, Medium BBa_J23108, High BBa_J23104) to control the expression level of the localisation tag. This enables optimisation of localisation for specific purposes. For example, when targeting a particularly large protein, it may be advantageous to use a lowly expressing promoter to prevent any disturbance to micro-compartment formation.
| Part Name | BioBrick | Description | Part Type |
|---|---|---|---|
| LowPromoter_PduD(1-20)_mCherry | BBa_K2213006 | Part Description | Basic |
| MediumPromoter_PduD(1-20)_mCherry | BBa_K2213007 | Part Description. | Basic |
| HighPromoter_PduD(1-20)_mCherry | BBa_K2213008 | Part Description. | Basic |
