Basic Molecular Biology Techniques:

PCR using Q5 Hi-Fi DNA polymerase Master Mix

PCR purification using QIAquick PCR purification kit

Gel extraction

Gibson Assembly

Digestion (NEB enzymes)

Ligation

Transformation of plasmids in E.coli DH5ɑ

Isolation of plasmid in E.coli DH5ɑ

Preparation of competent Lactococcus lactis

Electroporation for L. lactis

Plasmid DNA isolation from Lactococcus lactis

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Anti-microbial peptides assay:

Pre-experiment: CFU measurement

Inhibition ring

AMPs efficiency and MIC assay

Biofilm formation test

Growth curve measurement

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Nisin induction of gene expression in Lactococcus lactis:

Testing of S. aureus sensing pathway

Autolysis test