Difference between revisions of "Team:Austin UTexas/Demonstrate"
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| + | <p> Along with being one of the canonical amino acids utilized in protein synthesis, glutamate plays an important role as the main amino-group donor in the biosynthesis of nitrogen-containing compounds such as amino acids and nucleotides. Thus, we hypothesized that gadB overexpression via the P8 and P32 constitutive promoters and the high-copy number ColE1 origin induced a high metabolic load on the cells by shunting away glutamate from essential anabolic pathways. We believed that transformants containing the mutationally degraded gadB gene were selected for. In contrast, transformants containing the functional gadB gene were selected against due to having a depletion of glutamate substrates needed for important cellular processes. </p> | ||
Revision as of 05:01, 1 November 2017
Along with being one of the canonical amino acids utilized in protein synthesis, glutamate plays an important role as the main amino-group donor in the biosynthesis of nitrogen-containing compounds such as amino acids and nucleotides. Thus, we hypothesized that gadB overexpression via the P8 and P32 constitutive promoters and the high-copy number ColE1 origin induced a high metabolic load on the cells by shunting away glutamate from essential anabolic pathways. We believed that transformants containing the mutationally degraded gadB gene were selected for. In contrast, transformants containing the functional gadB gene were selected against due to having a depletion of glutamate substrates needed for important cellular processes.
