Difference between revisions of "Team:SUSTech Shenzhen/Contribution"
Jol-Fengzi (Talk | contribs) |
|||
| Line 7: | Line 7: | ||
{{:Team:SUSTech_Shenzhen/main-content-begin}}<!---------内容的样式----------------> | {{:Team:SUSTech_Shenzhen/main-content-begin}}<!---------内容的样式----------------> | ||
| − | To indicate neuron AWB in <i>C.elegans</i>’ head, we chose GFP for <i>Caenorhabditis elegans</i>. It’s the part BBa_K309016 submitted by iGEM10_Queens-Canada. However, the sample status shows that ”it’s complicated”, which means the sample may not have been sequenced or there is some problems on it. | + | To indicate neuron AWB in <i>C.elegans</i>’ head, we chose GFP for <i>Caenorhabditis elegans</i>. It’s the part <html><a href="http://parts.igem.org/Part:BBa_K309016">BBa_K309016</a></html> submitted by iGEM10_Queens-Canada. However, the sample status shows that ”it’s complicated”, which means the sample may not have been sequenced or there is some problems on it. |
{{SUSTech_Image_Center_fill-width | filename=T--SUSTech_Shenzhen--Charicterization1.png|width=1000px|caption=<B></B>}} | {{SUSTech_Image_Center_fill-width | filename=T--SUSTech_Shenzhen--Charicterization1.png|width=1000px|caption=<B></B>}} | ||
Revision as of 17:51, 1 November 2017
Characterization
Parts
To indicate neuron AWB in C.elegans’ head, we chose GFP for Caenorhabditis elegans. It’s the part BBa_K309016 submitted by iGEM10_Queens-Canada. However, the sample status shows that ”it’s complicated”, which means the sample may not have been sequenced or there is some problems on it.
We successfully improved characterization of GFP for C.elegans. We submit this optimized GFP as our part BBa_K2492000. We constructed a str-1::Chrimson::GEMgeco::GFP fusion gene and injected it into C.elegans. Promoter str-1 drives the expression of GFP in neuron AWB, which indicate the AWB location.
We capture the image of GFP specifically expressed in AWB with confocal microscope and clearly observe AWB with its dendrite(Figure).
