Difference between revisions of "Team:SUSTech Shenzhen/Contribution"
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To indicate neuron AWB in <i>C.elegans</i>’ head, we chose GFP for <i>Caenorhabditis elegans</i>. It’s the part <html><a href="http://parts.igem.org/Part:BBa_K309016">BBa_K309016</a></html> submitted by iGEM10_Queens-Canada. However, the sample status shows that ”it’s complicated”, which means the sample may not have been sequenced or there is some problems on it. | To indicate neuron AWB in <i>C.elegans</i>’ head, we chose GFP for <i>Caenorhabditis elegans</i>. It’s the part <html><a href="http://parts.igem.org/Part:BBa_K309016">BBa_K309016</a></html> submitted by iGEM10_Queens-Canada. However, the sample status shows that ”it’s complicated”, which means the sample may not have been sequenced or there is some problems on it. | ||
| − | {{SUSTech_Image_Center_fill-width | filename=T--SUSTech_Shenzhen--Parts-contribution.png|width=1000px|caption=<B>Figure 1. Parts information on BBa_K309016. A) The part registry page for BBA_K309016; B)：Description of ‘It’s Complicated’ in Registry. | + | {{SUSTech_Image_Center_fill-width | filename=T--SUSTech_Shenzhen--Parts-contribution.png|width=1000px|caption=<B>Figure 1. Parts information on BBa_K309016. A)</B> The part registry page for BBA_K309016; <B>B)</B>：Description of ‘It’s Complicated’ in Registry.}} |
{{SUSTech_Image_Center_8| filename=T--SUSTech_Shenzhen--Charicterization2.png|width=1000px|caption=<B>Figure 2. Alignment between BBa_K309016 and BBa_K2492000 (optimized GFP) at start codon.</B>}} | {{SUSTech_Image_Center_8| filename=T--SUSTech_Shenzhen--Charicterization2.png|width=1000px|caption=<B>Figure 2. Alignment between BBa_K309016 and BBa_K2492000 (optimized GFP) at start codon.</B>}} | ||
Revision as of 18:21, 1 November 2017
Characterization
Parts
To indicate neuron AWB in C.elegans’ head, we chose GFP for Caenorhabditis elegans. It’s the part BBa_K309016 submitted by iGEM10_Queens-Canada. However, the sample status shows that ”it’s complicated”, which means the sample may not have been sequenced or there is some problems on it.
We successfully improved characterization of GFP for C.elegans. We submit this optimized GFP as our part BBa_K2492000. We constructed a str-1::Chrimson::GEMgeco::GFP fusion gene and injected it into C.elegans. Promoter str-1 drives the expression of GFP in neuron AWB, which indicate the AWB location.
We capture the image of GFP specifically expressed in AWB with confocal microscope and clearly observe AWB with its dendrite(Figure.3).
