Difference between revisions of "Team:SUSTech Shenzhen/HP/Gold Integrated"

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Human practice activities are integrated throughout the design and execution of our project. Interaction with the public and fellow researchers led to many improvements in our design, like the design of behavior experiment, the improvement in microinjection procedures and better <i>C. elegans</i> mating method.</font>
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Human practice activities are integrated throughout the design and execution stages of our project. Interaction with the general public and scientific community led to many improvements in our design, such as the behavioral experiment, microinjection procedures and <i>C. elegans</i> mating method.</font>
 
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== Exchange of Ideas with Fellow Scholars==
 
== Exchange of Ideas with Fellow Scholars==
  
Considering our needs to design the behavioral experiment to train worms, we held a seminar with behavioristic psychology professor Peng Li of Shenzhen University, aiming to get some inspirations for our behavior experiment design.
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For the viability of the behavioral experiment design, we held a seminar with behavioral psychology professor Peng Li of Shenzhen University to get some professional advice.
  
In the seminar, Prof. Li gave some professional advise on our design in aspect of behavioristic psychology. Professor pointed out the lacking of an indifferent stimulus on which we can build a new behavior. He suggested building tropism on an indifferent factor by training worms with favorite/abhorrent stimulus. That was much easier than training worms to change their pre-existing likes and dislikes. The advice we got from Prof. Li helped us correct our principles and guided us to design our new behavior experiment.
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In the seminar, Prof. Li pointed out the lack of an indifferent stimulus on which we can build a new behavior. He also suggested building tropism on an indifferent factor by training worms with favorite or abhorrent stimulus. That was much easier than training worms to change their pre-existing likes and dislikes. The advice we got from Prof. Li helped us correct our principles and guided us to design our new behavioral experiment.
  
With the advice of Prof. Li, we amended our behavioral experiment design, preventing ourselves from wasting time.
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In such an interdisciplinary project, a smooth communication with experts of other fields can guarantee viability of our project and prevent ourselves from wasting time.
  
 
{{SUSTech_Image_Center_fill-width | filename=T--SUSTech_Shenzhen--HP-silver-8.png|width=6600px|caption=<B> 8.Communication with department of psychology and sociology SZU </B>}}
 
{{SUSTech_Image_Center_fill-width | filename=T--SUSTech_Shenzhen--HP-silver-8.png|width=6600px|caption=<B> 8.Communication with department of psychology and sociology SZU </B>}}
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{{SUSTech_Image_Center_fill-width | filename=T--SUSTech_Shenzhen--HP-silver-9.png|width=6600px|caption=<B> 9.Poster exhibition in CCIC</B>}}
 
{{SUSTech_Image_Center_fill-width | filename=T--SUSTech_Shenzhen--HP-silver-9.png|width=6600px|caption=<B> 9.Poster exhibition in CCIC</B>}}
  
After presenting our project on the conference, we had a short discussion with the judges, who provided us with much valuable advice. Judges raised concerns that the long-time training which may make <i>C. elegans</i> too big.  
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After presenting our project on the conference, we had a short discussion with the judges, who provide us with much valuable advice. Judges raised concerns that the long-time training may   result in old <i>C. elegans</i> and additional uncertainties. Therefore, we adapted their suggestions  and modified our training pattern and related experiment designs.
 
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Therefore, we verified this hypothesis and modified our training pattern and experiment design.
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{{SUSTech_Image_Center_fill-width | filename=T--SUSTech_Shenzhen--HP-silver-10.png|width=6600px|caption=<B> 10.Group Photo in CCIC</B>}}
 
{{SUSTech_Image_Center_fill-width | filename=T--SUSTech_Shenzhen--HP-silver-10.png|width=6600px|caption=<B> 10.Group Photo in CCIC</B>}}
  
In this conference, we were enlightened about the design of our experiment. This opportunity also provided us with new ideas about synthetic biology.
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In this conference, we were enlightened about the design of our experiment. This opportunity also gave us new ideas about synthetic biology.  
 
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== Collaboration with Fellow Researchers==
 
== Collaboration with Fellow Researchers==
  
At initial stage, we faced lots of problems on microinjection, such as low success rate and low male worms rate after mating. So we contacted a laboratory which research mainly in <i>C. elegans</i> area in HKBU(Hong Kong Baptist University) with the help of Prof.Huang. We communicated with Doctor Ding on microinjection issues in the lab. With their kind instruction on our microinjection methods, we got our MiniMos part successfully inserted into <i>C. elegans</i>.
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At initial stage, we faced lots of problems on microinjection, such as low success rate and low male worms rate after mating. So we contacted Prof. Zhongying’s laboratory at Hong Kong Baptist University, one of the best in genetic manipulation of <i>C. elegans</i> area. We communicated with Doctor Ding on microinjection issues in Prof. Zhao’s lab. With their kind and patient instructions on our microinjection methods, we successfully inserted our parts into <i>C. elegans</i>.
  
 
{{SUSTech_Image_Center_fill-width | filename=T--SUSTech_Shenzhen--HP-silver-11.png|width=6600px|caption=<B> 11: Microinjection Discussion in HKBU</B>}}
 
{{SUSTech_Image_Center_fill-width | filename=T--SUSTech_Shenzhen--HP-silver-11.png|width=6600px|caption=<B> 11: Microinjection Discussion in HKBU</B>}}

Revision as of 22:12, 1 November 2017

Team SUSTC-Shenzhen

Human Practices

Our step to make a better world


Human practice activities are integrated throughout the design and execution stages of our project. Interaction with the general public and scientific community led to many improvements in our design, such as the behavioral experiment, microinjection procedures and C. elegans mating method.


Exchange of Ideas with Fellow Scholars

For the viability of the behavioral experiment design, we held a seminar with behavioral psychology professor Peng Li of Shenzhen University to get some professional advice.

In the seminar, Prof. Li pointed out the lack of an indifferent stimulus on which we can build a new behavior. He also suggested building tropism on an indifferent factor by training worms with favorite or abhorrent stimulus. That was much easier than training worms to change their pre-existing likes and dislikes. The advice we got from Prof. Li helped us correct our principles and guided us to design our new behavioral experiment.

In such an interdisciplinary project, a smooth communication with experts of other fields can guarantee viability of our project and prevent ourselves from wasting time.

T--SUSTech Shenzhen--HP-silver-8.png
8.Communication with department of psychology and sociology SZU


Communication with Other Teams

During 26th,Aug and 28th,Aug this year, our team was invited to attend CCiC (Conference of China iGEMer Committee) in Fuzhou, China, to present our project among peers researchers.

All the 49 teams invited to the conference gave presentations and exhibited posters about their projects. During the conference, especially in the poster session, we had many opportunities to communicate with other teams and exchange ideas of our project designs.

T--SUSTech Shenzhen--HP-silver-9.png
9.Poster exhibition in CCIC

After presenting our project on the conference, we had a short discussion with the judges, who provide us with much valuable advice. Judges raised concerns that the long-time training may result in old C. elegans and additional uncertainties. Therefore, we adapted their suggestions and modified our training pattern and related experiment designs.

T--SUSTech Shenzhen--HP-silver-10.png
10.Group Photo in CCIC

In this conference, we were enlightened about the design of our experiment. This opportunity also gave us new ideas about synthetic biology.


Collaboration with Fellow Researchers

At initial stage, we faced lots of problems on microinjection, such as low success rate and low male worms rate after mating. So we contacted Prof. Zhongying’s laboratory at Hong Kong Baptist University, one of the best in genetic manipulation of C. elegans area. We communicated with Doctor Ding on microinjection issues in Prof. Zhao’s lab. With their kind and patient instructions on our microinjection methods, we successfully inserted our parts into C. elegans.

T--SUSTech Shenzhen--HP-silver-11.png
11: Microinjection Discussion in HKBU
T--SUSTech Shenzhen--HP-silver-12.png
12: Learning Microinjection
T--SUSTech Shenzhen--HP-silver-13.png
13: Pick worms with a bamboo stick


Made by from the elegans.Inc in SUSTech_Shenzhen.

Licensed under CC BY 4.0.