Team:SUSTech Shenzhen/Hardware

Hardware

Model

1. Microfluicds

2. Optical Devices

2.1 Light Path Design

We need 395nm light to activate GEM-GECO by Lumencor LED light Soures, then both red light and blue light activate CoChR or Chrimson by LCD projecter. LCD projector and LED light source are combined by a tee which uses a semi-transparent mirror. Tee connect to microscopy light entrance. So we can use these two light together.

Fig. 2 Light Pathway of projecter and microscope

1000px

Fig. 3 Red, blue, green out from projecter

Because the focus distance of origin lens in projector too short for microscopy, a longer focus distance lens is replaced. We install a new lens in projector.

To purify light form projector, origin blue channel filter is replaced in [Chroma ET480/20X](https://www.chroma.com/products/parts/et480-20x) and red channel filter is replaced in [Chroma ET630/20X](https://www.chroma.com/products/parts/et630-20x). Dimirror is [89402bs](https://www.chroma.com/products/parts/89402bs), and emssion is [89402m](https://www.chroma.com/products/parts/89402m).

| Bule Channel | Red Channel | Dimirror | Emssion filter for GEM-GECO | |----------------|----------------|------------|-----------------------------------| | Chroma ET480/20X | Chroma ET630/20X | Chroma 89402bs | Chroma 89402m |

Reference

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