Team SUSTC-Shenzhen

Interlab

Contribution

Interlab Study

Description

Reliable and repeatable measurement is a key component to all engineering disciplines including synthetic biology. However, the ability to repeat measurements in different labs has been difficult.This year the interlab study is aim to improve the measurement tools available since the fluorescence data usually cannot be compared because it is reported in different units or because different groups process data in different ways. The InterLab protocol aims to address these issues by providing the teams with a detailed protocol and data analysis form that yields absolute units for measuring GFP in a plate reader.</br>

Material&Methods

Protocal link </br> </br>

Results

T--SUSTech Shenzhen--Microfuildics--gsp.png

Fig.1-1 Fluorescein Standard Curve

Fig.1-2 Fluorescein Standard Curve (log scale)

Fig.2-1 The ratio of uM Fluorescein/OD600, which convert the Fluorescence intensity of each test device or control group(Colony 1) to the number of Fluorescein.

Fig.2-2 The ratio of uM Fluorescein/OD600, which convert the Fluorescence intensity of each test device or control group(Colony 2) to the number of Fluorescein.

Conclusion

It can be seen that for some devices have obvious errors which indicating a lot of variation in the results. In general, the ratio decreased over time for all samples.</br>

During the experiment ,the result is better when culture the cell in  amber falcon tube than that in the normal tube covered with foil.
The standard curve is not very linear, some data made the curve a little crooked.</br>
The plasmid test device 1 had the problem to transformation and is difficult to culture the E.coli contained it. We have sequenced all the plasmids and compared with the map on the item official web site , then we found out that the sequence of the bacteria are not all correct. So we do a series selection to picked the right one out. However, the colony device 1 with correct sequence may have much more expression the result is not very idea, as well.

Team:SUSTech Shenzhen/InterLab