Team SUSTC-Shenzhen

Hardware

Maker for wisdom of Life

1. Hardware

T--SUSTech Shenzhen--Microfluicds&Microscope.jpg

a

1. Mircofluicdcs

2. Design for Light

2.1 Basic of mechanical and optics

We need 395nm light to activate Calcium indicator protein GEM-GECO by Lumencor LED Illuminator. Then both red light and blue light activate CoChR or Chrimson by LCD projector. LCD projector and LED Illuminator are merged by a double LH Adapter contained a semi-transparent mirror. Double LH Adapter connect to entrance of light in microscope. So we can use these two light source together.

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Fig. 2Light Pathway of projecter and microscope

We choose projector as red and blue light source, because we can activate single cell or C. elegans with adjustable color and strength.

Fig. 3 Red, blue, green out from projecter

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Because the focus distance of origin lens in projector too short for microscopy, a longer focus distance lens is replaced. We install a new lens in projector.

Fig 5. Filter and mirrors in our system

To purify light form projector, origin blue channel filter is replaced in Chroma ET480/20X and red channel filter is replaced in Chroma ET630/20X. Dimirror is 89402bs, and emssion filter is 89402m.

=== 2.2 Time

Team:SUSTech Shenzhen/Hardware

Hardware

Contents

1. Hardware

1. Mircofluicdcs

2. Design for Light

2.1 Basic of mechanical and optics

References