Team:SUSTech Shenzhen/Hardware
Hardware
Create for wisdom of Life
Contents
1. Mircofluicdcs
Under construction
2. Play with light in spatio-temporal
Multiple devices of optics are designed and created for the various requirement of experiments. All devices are attempt to modulate the spatio-temporal pattern elegantly and effectively. Just let us start to play with light.
2.1 Device One: Arduino modulate Mercury lamp
A simple and effective device to output pulse of certain wavelength of light. On time and off time of pulse is custom by Arduino. Wavelength of light is modified by replacing filter before beam expander.
2.2 Device Two: Projector Tracker
Here is a more fantastic and powerful tool with a LCD Projector we design.
2.2.1 Basic mechanical and optics
We need 395nm light to activate Calcium indicator protein GEM-GECO by Lumencor LED Illuminator. Then CoChR and Chrimson are activated by blue light and red light from LCD projector. LCD projector and LED Illuminator are merged by a double LH Adapter contained a semi-transparent mirror. Double LH Adapter connect to entrance of light in microscope. So we can use these two light source in the same time.
We choose projector as red and blue light source, because we can activate single cell or C. elegans by light of adjustable color and intensity. Because the focus distance of origin lens in projector too short for our microscopy(Nikon Ti-E), a longer focus distance lens is replaced. We install a new lens into projector. Smaller the image of projector, and put image into microscope.
Protein are sensitive to the wavelength of light, it necessary to purify light of the projector. We buy and replace with new filter Chroma ET480/20X and Chroma ET630/20X, both are fixed inside projector. Di-mirror 89402bs and emission filter 89402m are been installed in Microscope filter wheel.
References
