Team:SUSTech Shenzhen

SUSTech_Shenzhen

It has been proved that C. elegans AWA and AWB neurons responsible for love and hate in the olfactory feelings, based on this background, our team hopes to explore the learning behavior of C. elegans through light genetic method. Using miniMos transfection, we build plasmids expressing specific photoreceptors and calmodulins in AWA and AWB. When giving excitation light in different wavelength (corresponding to different photoreceptor protein), light-sensitive proteins can receive stimuli and cause corresponding calcium ion concentration changes, indirectly influence calmodulins changing the light. Based on this assumption, we plan to use microfluidic methods to determine whether worms are capable of learning. For the odors that the C. elegans likes, we plan to use repeated AWB neuron light stimulation to see whether worms can learn the ability of dislike. So is for AWA. If we can demonstrate the ability of learning, we will further study the downstream protein pathways of AWA and AWB, trying to decipher the network of learning behaviors in the olfactory nerve.

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