Team:Lethbridge/Design
Design Objectives
Our design was guided by the needs of three user groups:
Simple scientific protocols
Standardized parts
Well characterized and developed system
Standard, easy to use system
Simplified protocols
Modular tool that can be tailored to the application
Modular experiments
Open-source
Open-source
Promote good safety practices
Standardized parts
Resulting in the identification of four core design considerations for our system
STANDARD, MODULAR, SAFE, and USER-FRIENLDY
Overview
To ensure utility of our system, we developed a simplified expression and purification strategy to produce the necessary biomachinery for cell-free synthetic biology.
Our system includes all of the 36 essential TX-TL proteins, the 23S and 16S rRNA, and a tRNA for each amino acid, where tRNAPhe will act as a proof of concept for our novel purification method. In addition, we include the MS2 coat protein which is essential to our tRNA and rRNA purification strategies.
Standard Construct Design
Purification Strategy
tRNA
Ribosomes
Validation Construct
Next vivo 2.0
